Culture Methods,Solid Medium,Liquid Medium,Optimum Growth,Suspension of Bacteria,Liquid Cultures,Microorganism

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Microbes and Microbial Genomics for Industry Microbial and Microbial Genomics for Industry - Introduction Isolation and Culturing of Micro-organisms Isolation Methods Sterilization and Disinfection Methods Culture Methods Production of Organic Compounds by Microbial Fermentation Ethanol Production by Microbial Fermentation Preparation of Substrate by Microbial Fermentation Acetone Butanol Production by Microbial Fermentation Gluconic Acid Production by Microbial Fermentation Some Organic Compounds Produced by Microbial Fermentation Production of Other Organic Compounds by Microbial Fermentation Production of Enzymes by Micro Organisms Activity Based Approach for Microbial Biocatalysts by Micro Organisms Production of Alpha Amylases by Micro Organisms Production of Proteases by Micro Organisms Production of Lipases by Micro Organisms

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Culture methods
Most of the industrially useful bacteria and fungi are cultured on either a solid medium or a liquid medium with a support. In either case, the medium should contain all essential ingredients needed for optimum growth of that particular micro-organism. Solid medium is generally prepared by melting agar at 100°C (in water) and then solidifying it at about 45°C. The medium which is still in a molten state, is poured into a suitable container and is allowed to cool and solidify. It is then inoculated by dipping a needle into the suspension of bacteria or fungi and drawing this needle right across the surface of the medium. After a few days of inoculation, colonies of micro-organism appear on the surface of the medium. In liquid cultures, all essential nutrients are dissolved in water, and no solidifying agent like agar is needed.

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