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Home >> Industrial and Microbial Biotechnology >> Microbes and Microbial Genomics for Industry >> Acetone Butanol Production by Microbial Fermentation

Acetone/butanol production
Acetone, butanol, butyric acid and isopropanol are obtained through fermentation of starch, molasses, wood hydrolysates and pentoses using bacteria belonging to the genus Clostridium. The relative proportion of different products varies with the species and the strain of Clostridium used. The biosynthetic steps involved in the production of each of these compounds are interrelated, all of them being derived from acetyl CoA produced from either glucose or fatty acid degradation.

Pathway of biosynthesis of acetic acid, butanol, acetone and isopropanol

An outline of the Pathway of Biosynthesis of Acetic Acid, Butanol, Acetone and Isopropanol.


In a typical fermentation process, the substrate includes molasses plus maize steep liquor, which are taken in a suitable container which is gassed with CO2 The fermentation is carried out at 33-35°C, with initial pH of 5.8 to 6.0. However, the pH drops in the first 18 hours increases again due to the production of acetone and butanol from these acids. Contamination due to bacteriophages and lactobacilli is a common problem in such a fermentation and therefore absolute sterilization is necessary.

In India, alcohol produced from molasses is used as a feedstock for production of acetone is used per MT of butanol and 2.2 MT of alcohol is used per MT of acetone. Acetone, butanol and ethanol are recovered through continuous distillation and fractionation. Carbon dioxide produced is recovered and converted into liquid CO2 or dry ice. The residue left after the distillation may be dried and utilized as animal feed.

 

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