Completion of Partial Pathways Giving New Products,Natural Steins,2-Keto-L-Gluconic Acid,Ascorbic Acid,2,5-Diketo-D-Gluconic Acid

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Your Ad Here	Completing of partial pathways giving new products Natural strains of any organism may be imperfect from an applied perspective and can be improved by extension of native pathways. Some of the example, summarized in include the following : (i) 2-keto-L-gluco-nic acid (2-KLG) is a precursor of ascorbic acid (vitamin c) and its synthesis involves two steps, first involving conversion the conversion of glucose to 2,5-diketo-D-gluconic acid (2,5-DKG) in Erwinia herbicola, and second involving the conversion of 2,5-DKG to 2-KLG in a species of Corynebacterium.
It has been shown that by cloning and transfer of gene for 2,5-DKG reductase from Corynebacterium to Erwinia, glucose can be converted to 2-KLG in a single fermentation step, (ii) A similar approach (as for 2-KLG) was used for the synthesis of 7-aminocephalosporanic acid (7 ACA), which can be used as the precursor for several semi-synthetic antibiotics in Acremonium chryosgenum. (iii) A glycoprotein like erythropoietin produced by Chinese hamster ovary (CHO) cells can be subjected to posttranslational modification by the transfer of a gene for β-galactoside α 2,6 sialyl-transferase.
Completion of Partial Pathways Giving New Products	Your Ad Here
This enzyme causes sialyl α 2,6,-galactosyl linkage on surface glycoprotein and can convert erythropoietin into a kind more closely resembling human erythropoietin which is rich in these linkages. (iv) Human H blood group antigen could be produced in mouse cells due to transfection with human DNA.