Cloning and Expression of Heterologous Genes,Erwinia Herbicola,Acremonium,Chrysogenum,Cephalosporin,Corynebacterium,Fusarium Salani

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Metabolic Engineering and Metabolomics Metabolic Engineering and Metabolomics Introduction Cloning and Expression of Heterologous Genes Completion of Partial Pathways giving new Products Transfer of an Entire Biosynthetic Pathway Creating new Products and new Reactants Altering Nutrient uptake and Metabolite Transfer of Promising Natural Motifs Redirecting Metabolite Flow Desensitizing Feedback Inhibition Increased Threonine in Bacteria Increased Lysine Content in Crop Plants Elevation of the Activity of rate Limiting Enzymes Alteration in Protein Processing Pathway Reduction of Competition for Limited Resources Modification of Metabolic Regulation Molecular Breeding of Biosynthetic Pathways

Home >> Industrial and Microbial Biotechnology >>Metabolic Engineering and Metabolomics >> Cloning and Expression of Heterologous Genes

Cloning and Expression of Heterologous Genes
Introduction of foreign gene(s) may lead to expression of a heterologous enzyme or transport system in. any organism leading to the production of new compounds. However, these new compounds may undergo further reactions
Heterologus activities recruited to alter metabolite and protein and products.

Hostorganism	Original metabolite	Heterologous enzyme added (source organism)	New product
1. Erwinia herbicola	2, 5-DKG	2, 5-DKG-reductase (Corynebacterium)	2-KLG
2. Acremonium chrysogenum	Cephalosporin C	(i) D-amino acid oxidase (Fusarium salani) (ii) Cephalosporina acylase (Pseudomonas diminuta)	7 ACA
3. CHO cells	Terminal β galactosyl residues in N-acetyllactosamine sequences	β-Galactoside α 2, 6 sialyl-transferase (rat)	Sialyl α 2, 6 galactosyl linkages
4. Mouse L cells	Unsubstituted type II, N-acetyllactosamine glycoconjugate end groups	G-Dp-L-fucose : β-D-galactoside 2-α-L-fucosyl-transferase (A431 human cell line)	H fucosyl α 1 2 galactosyl linkages

Which can not be anticipated, thus putting a limitation on its use for metabolic engineering. Further, a protein thus synthesized may or may not fold properly and may not be suitably localized. Despite these limitations, the number and scope of positive experiments encourage further application of this approach. Following are some of the possible applications of cloning and expression of foreign genes in metabolic engineering.

	Home
Carotenoid Biosynthesis Biosynthesis of Novel Carotenoids Biosynthesis of Acyclic and Cyclic Carotenoids Metabolic Engineering for PHAs Metabolic Engineering for Alkaloid Biosynthesis Pathway Analysis for Metabolic Engineering Metabolic Control Analysis (MCA) for Metabolic Engineering Metabolomics and Metabolic Engineering MS,NMR and Metabolomic Profiling Metabolomic Control Analysis and FANCY Limitations in Metabolic Engineering Due to Technology Metabolic Control Theory and Metabolic Engineering Limitations in Metabolic Engineering Due to Network Rigidity Identification and Classification of Principal Nodes Assessment of Nodal Rigidity and its Response to Metabolic Engineering

Language >> English | Français | Español | Deutsch | Italiano | Portugues | Japanese | Korean | Sim-Chinese