Specially designed columns for EBA are also available commercially (e.g. Streamline from Amersham Pharmacia, Sweden). A number of enzymes/ proteins that have been successfully purified by EBA include glucose-6 phosphate dehydrogenase, polyphenol oxidase, β-D galactoxidase, bovine serum albumin, endostatin, etc. It has been shown that if an affinity ligand is used with EBA, expanded bed affinity chromatography (EBAC) will be much more efficient than the simple EBA. Following are two examples, where such an affinity ligand is used with EBA.

Purification of cellulase on chitosan beads. Cellulase are often found associated with β-glucosidase, and chitosan beads have been used to obtain cellulases (from Aspergills niger and A. fumigatus) that are free  of β- glucosidase. Cellulose beads (called CELBEADS) have also been used for the purification of cellulase, and many cellulose beads are known, although their use in expanded bed chromatography has not been evaluated. In future, cellulose beads will certainly be used for purification of cellulases and other fusion proteins having cellulose binding domain tag.