Enzyme Engineering, Immobilized Enzymes

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Biocatalysis and Enzyme Biotechnology Biocatalysis and Enzyme Biotechnology - Introduction Some Research Activities Involving Enzymatic Synthesis Properties of Enzymes Parameters Affecting Enzymatic Reactions Definitions of Some Parameters used for Describing Key Properties of Enzymes Medium for Enzymes Aqueous vs Organic Solvent Selectivity of Enzymes Types of Enzymes Biomimetic Catalysts Enzymes Commonly Used in Organic Synthesis Extremozymes Cofactor Dependent Enzymes and Cofactor Regeneration Modular Enzymes Coenzymes Use of Enzymes Medical and Clinical Uses Drugs for Digestive Disorders Industrial Uses Uses of Industrial Uses Textile Industry Uses of Leather Industry Uses of Paper Industry Cellulase Free Xylanases Uses of Food Industry Isolation and Purification of Enzymes

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Enzyme Engineering
Enzymes can also be specifically engineered to produce forms not available in nature. These will then meet specific needs of commercial application of immobilized enzymes.They are
(i) Protein For Enzyme Engineering
(ii) Engineering For Macromolecules

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Extraction of Enzymes Preparation of Crude Enzymes Purification of Enzymes Chromatography Adsorption Chromatography Ion Exchange Chromatography Gel Filtration Chromatography Affinity Chromatography Electrophoresis Immobilization of Enzymes or Whole Cells Techniques for Immobilization Adsorption Method Covalent Binding Method Cross Binding Method Some Reagents Used to Immobilize Enzymes by Cross Linking Entrapping Method Entrapping by Microencapsulation Requirements for Commercial use of Immobilized Enzyme Systems Enzyme Carrier Systems Some Applications of Immobilized Enzymes and Whole Cells Reactor Types Operating Strategy Stability of Immobilization and Protection of Immobilized EnzymesCells Some Examples of the Use of Immobilized Enzymes Cells Industrial Biocatalysis Biotransformation of Polyunsaturated Fatty Acids PUFAs Hydrolalse Mediated Biotransformations Oxidative Biotransformation Enzyme Engineering