Entrapping Method, Polymerization, Substrate Molecules, Polyacrylamide, Silicone Rubber Gel, Immobilization, Entrapment Method

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Entrapping Method
Enzymes can be entrapped inside a cross-linked gel matrix by allowing the gel to be formed in an aqueous solution containing one or more enzymes. The polymerization of the gel is carried out in the presence of enzyme(s). The enzyme is physically entrapped within the matrix and cannot escape by permeation.

Entrapping of Enzyme Inside a Polymerized Gel

However, substrate molecules, being smaller can defuse in, can be acted upon by enzyme and can then diffuse out of the matrix. The important matrices for entrapping enzymes are polyacrylamide, starch and silicone rubber gel. However, immobilization of enzymes by such an entrapment method is often associated with following three types of difficulties: (i) There is continuous leakage of enzyme because of wide pore size distribution in the gel. (ii) There is reduced substrate accessibility to the enzyme. (iii) There is some loss of enzyme activity due to free radicals produced during polymerization of the gel.

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