Definitions of Some Parameters used for Describing Key Properties of Enzymes, Fluorescence, Calorimetry

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Biocatalysis and Enzyme Biotechnology Biocatalysis and Enzyme Biotechnology - Introduction Some Research Activities Involving Enzymatic Synthesis Properties of Enzymes Parameters Affecting Enzymatic Reactions Definitions of Some Parameters used for Describing Key Properties of Enzymes Medium for Enzymes Aqueous vs Organic Solvent Selectivity of Enzymes Types of Enzymes Biomimetic Catalysts Enzymes Commonly Used in Organic Synthesis Extremozymes Cofactor Dependent Enzymes and Cofactor Regeneration Modular Enzymes Coenzymes Use of Enzymes Medical and Clinical Uses Drugs for Digestive Disorders Industrial Uses Uses of Industrial Uses Textile Industry Uses of Leather Industry Uses of Paper Industry Cellulase Free Xylanases Uses of Food Industry Isolation and Purification of Enzymes

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Definitions of Some Parameters used for Describing Key Properties of Enzymes

Parameter	Definition
T_m (melting temp)	Temperature at which 50% denaturation of enzyme occurs (determined by fluorescence, circular dichroism (CD) or calorimetry)
T_opt (optimum temp)	Temperature for optimum enzyme activity for a given set of reaction conditions
K_s and K_p (dissociation constants for substrate and product)	Measure of dissociation; low values cause inhibition
K_cat (turnover number or rate constant)	Maximum number of substrate molecules converted to product per unit time per active site; a function of rate constants for the conversion of enzyme-substrate (ES) complex to enzyme-product complex
K_M (Michaelis constant)	Represents dissociation of the ES complex, indicating substrate binding to active site
K_cat/K_M (specificity constant)	Indicates rate of association of enzyme and substrate and also the specificity for competing substrates

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