Antisense Inhibition Using PNA,Antisense Technology,Antisense RNA,Hybridization Properties,Enzymatic Degradation,Ribosome,RNA Polymerases

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Triplex DNA, TFOs, PNAs, RNA-DNA Hybrids and dsRNA - RNAi Triplex DNA, TFOs, PNAs, RNA-DNA Hybrids and dsRNA - RNAi - Introduction Triplex DNA and TFOs H Form or H DNA PNAs Peptide Nucleic Acids PNA DNA and PNA RNA Hybrid Complexes Synthesis of PNAs by BOC or Fmoc Protocols Modification of PNAs for Specific Uses

Home >> Biotechnology and Genomics >> Triplex DNA, TFOs, PNAs, RNA-DNA Hybrids and dsRNA - RNAi >> Antisense Inhibition Using PNA

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Antisense inhibition using PNA.
Antisense RNA hybridizes with mRNA and does not allow translation, so that gene expression can be inhibited through the use of antisense technology. In this antisense technology, PNA can be substituted for antisense RNA, since PNA has superior hybridization properties, is resistant to enzymatic degradation, and can be variously modified at the chemical level. The stability of PNA, when bound to complementary DNA or RNA sequence causes hinderance in the functioning of ribosome and several enzymes including DNA and RNA polymerases, reverse transcriptase and telomerase, thus giving it an edge over RNA in antisense technology. In E. coli, it has been shown that PNA targeted to specific gene sequences, inhibits gene expression, thus opening possibilities of using this technology for antibacterial purposes and for other similar uses.

For instance, in a report published in 2001; antisense PNA oligomers were shown to inhibit expression of episomal/chromosomal genes in cultured trophozoites of Entamoeba histolytica, the causative of Entamoeba histolytica, the causative agent world-wide for amoebiasis in 500 million people every year. The antisense PNA in this report was also shown to check cellular proliferation and cell growth in trophozoites belonging to E. histolytica suggesting that it can be used for a control of the disease amoebiasis. Regulation of gene expression by antisense technology using PNAs may also find applications in fermentation industry and for study of gene functions as a part of functional genomics.

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