Polymerase Chain Reaction PCR and Gene Amplification Introduction, DNA, Gene Cloning, Prokaryotic, Eukaryotic Cell, Genetic Engineering, Molecular Biology

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Polymerase Chain Reaction-PCR and Gene Amplification Polymerase Chain Reaction - PCR and Gene Amplification - Introduction Basic Polymerase Chain Reaction - PCR Comparison of Polymerase Chain Reaction and Gene Cloning Different Schemes of Polymerase Chain Reaction Inverse Polymerase Chain Reaction Anchored Polymerase Chain Reaction Polymerase Chain Reaction for Site Directed Mutagenesis Cloning Expression and Sequencing of the Polymerase Chain Reaction Product

Home >> Biotechnology and Genomics >> Polymerase Chain Reaction-PCR and Gene Amplification >> Polymerase Chain Reaction PCR and Gene Amplification Introduction

Your Ad Here

Polymerase Chain Reaction PCR and Gene Amplification Introduction

The techniques of recombinant DNA and gene cloning, which permit us to obtain an unlimited supply of identical copies of a gene sequence or DNA segment, which is cloned in a prokaryotic or a eukaryotic cell with the help of a vector. This technique, discovered around 1975, proved extremely useful in all experiments of molecular biology and genetic engineering and thus became an essential tool in all molecular biology laboratories.

In 1985, yet another remarkable tool in molecular biology was discovered, which is known as polymerase chain reaction (PCR), later nick named as 'people's choice reaction'. The methodology is so important that the prestigeous journal Science published from USA considered PCR as the major scientific development of the year 1989, and had chosen Taq DNA polymerase, the enzyme used in PCR, as the molecule of the year 1989.

Kary Mullis, who discovered this reaction in 1985, described in his article published in April 1990 issue of Scientific American, how he got the basic idea of this reaction, while driving through the red wood mountains of California, USA. In view of the significance that PCR and its varied applications achieved within a few years after its discovery, the 1993 Nobel Prize in Chemistry was shared by Karry Mullis for his discovery of PCR, with Michael Smith, for his discovery of site directed mutagenesis.

The polymerase chain reaction is such a powerful technique that for majority of laboratory experiments, it has now largely replaced the gene cloning undertaken with the help of vectors. The basic principles and the methodology involved in PCR (including its various modified forms) and its various uses in molecular biology and biotechnology will be discussed in this.

	Home
Asymmetric Polymerase Chain Reaction for DNA Sequencing Reverse Transcribed-RT Polymerase Chain Reaction Application of Polymerase Chain Reaction in Biotechnology and Genetic Engineering Gene Tagging Using Polymerase Chain Reaction Polymerase Chain Reaction for Confirming the Presence of Transferred Gene Human Genetics Using Polymerase Chain Reaction Recombination Data Using Polymerase Chain Reaction Sexing of Embryos Using Polymerase Chain Reaction DNA Fingerprinting Using Polymerase Chain Reaction Electronic Polymerase Chain Reaction-ePCR