Cloning, Expression and sequencing of the PCR product
The amplified PCR product can be cloned, if restriction sites for specific enzymes are introduced at the 5' end of each primer. These restriction sites do not interfere with amplification and can be used for cloning with a variety of vectors in the usual manner. Similarly, incorporation of a T7 promoter at the 5' end of one primer will allow the use of RNA polymerase enzyme to get RNA copies of PCR product.

PCR amplified products can also be cloned, without using modified primers in PCR reactions. For this purpose the amplified product is purified using QIA quick PCR purification kit (a product of QIAGEN, avialable from Genetix, New Delhi) and ligated to Promega's pGEM-T or pGEM-T Easy vector. The chimeric vector .is multiplied in E.coli cells and the insert (PCR product) is sequenced using universal M13 primers.