Application of PCR in Biotechnology and Genetic Engineering

During 1990-2000, with the improvement of PCR protocols and, due to the availability of automatic thermal cyclers commercially, the applications of PCR have increased manyfold. However, for application of PCR, we need a pair of primers which should be based on the knowledge of nucleotide sequence of DNA to be amplified. Therefore, non-availability of this information about the DNA segment or gene to be amplified. becomes a limitation in the applications of PCR, although this difficulty has been overcome for the study of DNA polymorphism, through the use of random DNA primers. Some of the applications of PCR technology , while describing molecular markers.