Methods for Whole Genome Sequencing, DNA Fragmetns, DNA Synthesis, Dideoxynucleoside Triphosphates, Genome Sequence

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Methods and Uses of Genomics and Proteomics Research Methods and Uses of Genomics and Proteomics Research - Introduction Genomics Research Methods for Whole Genome Sequencing Whole Genome Shotgun-WGS Sequencing Map First Sequence Later or Clone by Clone Strategy Construction of Whole Genome BAC Map / BAC-By-BAC Approach Building of Clone Contigs / Overlapping Series of Cloned DNA Fragments Whole Genome Sequence Data Electronic PCR (e-PCR) / Bridging the Gaps Between Mapping and Sequencing of Genomes From Genome Sequences to Function - Annotation

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Methods for whole genome sequencing

Methods for rapid and efficient sequencing of DNA fragment were developed in 1970s. Which were described. The chain termination DNA synthesis involving ddNTPs (dideoxynucleoside triphosphates) developed by Frederick Sanger has been preferred over other methods for genome sequencing. Automated sequencers have also been developed to accelerate the rate at which sequencing data can now be generated. More recently new automated sequencers have been introduced, which makes use of capillary separation rather than polyacrylamide gel separation of small synthesized DNA fragments.

These new generation automated sequencers (e.g. ABM3700; MegaBACE 4000), each has 96 or even upto 384 channels thus permitting determination of 96 or 384 sequences in parallel. Since each run takes less than 2 hours, 1000 to 4000 sequences can be obtained from a single sequencing machine in one day. This method is now routinely used for shotgun sequencing of BAC clones, as discussed in the next section

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Methods for Annotation of Genome Sequences Annotation By Sequence Search Loss of Function by Mutation Approach Proteomics Research Significance of Proteomics Research Methods of Proteome Analysis Resolution and Identification of Proteins Mass Spectrometry Comparative 2D-gel Approach Protein Chips Approach Analysis of Post Translational Modification of Proteins Protein-Protein Interactions Purification of Protein Complexes Phage Display Yeast Two Hybrid System Functions of all the Ptoteins of Yeast Worked Out