Mass Spectrometry, Gel-Separated Proteins, Peptides, Proteases, Trypsin

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Methods and Uses of Genomics and Proteomics Research Methods and Uses of Genomics and Proteomics Research - Introduction Genomics Research Methods for Whole Genome Sequencing Whole Genome Shotgun-WGS Sequencing Map First Sequence Later or Clone by Clone Strategy Construction of Whole Genome BAC Map / BAC-By-BAC Approach Building of Clone Contigs / Overlapping Series of Cloned DNA Fragments Whole Genome Sequence Data Electronic PCR (e-PCR) / Bridging the Gaps Between Mapping and Sequencing of Genomes From Genome Sequences to Function - Annotation

Home >> Biotechnology and Genomics >> Methods and Uses of Genomics and Proteomics Research >> Mass Spectrometry

Your Ad Here

Mass spectrometry

Mass spectrometric identification of gel-separated proteins is commonly used for further analysis of a proteome. Since whole proteins are difficult to elute and analysed by mass spectrometry, the gel separated proteins are digested into peptides, by one or more sequence specific proteases (e.g. trypsin), so that these trypsinated peptides can then be separated and analysed by mass spectrometry. The different approaches for mass spectrometric analysis of proteins

Differential Display

Much information about the functions of genes and proteins is derived from the analysis of mRNA expression profiles, gene disruption phenotypes, two hybrid interactions, and by subcellular localization of proteins. But these strategies do not give information about the function of proteins at the biochemical level. In biomedical applications, often the objective is to identify proteins that are up-or down-regulated in a disease specific manner, so that these proteins may be used for diagnosis and/or for identification of therapeutic targets. This is achieved by a study of differential display of certain proteins, either by comparative 2-D gel approach or by using protein chips.

	Home
Methods for Annotation of Genome Sequences Annotation By Sequence Search Loss of Function by Mutation Approach Proteomics Research Significance of Proteomics Research Methods of Proteome Analysis Resolution and Identification of Proteins Mass Spectrometry Comparative 2D-gel Approach Protein Chips Approach Analysis of Post Translational Modification of Proteins Protein-Protein Interactions Purification of Protein Complexes Phage Display Yeast Two Hybrid System Functions of all the Ptoteins of Yeast Worked Out