Electronic PCR (e-PCR) Bridging the gaps between mapping and sequencing of genomes)

With the availability of high density genome maps and large scale genome sequencing, data are now available, which can be used for finding out the expected size of PCR product through the use of a specific pair of primers, This can b don electronically on the computer without running t wet PCR reactions without running the wet PCR reactions, hence the name e-PCR (consult details).

Mapped STS or SSR loci can be searched in the genomic DNA sequences available in the databses and matched sequences can be placed on the physical maps using the corresponding positions of mapped STS/SSR loci. This has been successfully done for human genome and becomes an important strategy to deal with genomic DNA sequence entries in the database, for which map positions are not available. This approach of e-PCR will be extensively used in future as more genome sequences become available in the database.