Thermospray, Particle Beam, Supersonic Jet, Small Droplets, Monodisperse Aerosol Generation Interface, Chromatography

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Home >> Biotechnology and Genomics >> Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis >>Thermospray and Particle Beam (PB)

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Thermospray and particle beam (PB)
Thermospray ionization involves coupling of HPLC to mass spectrometry at conventional flow rate (0.5 to 1.5 ml/min). the effluent from HPLC column is vaporized under reduced pressure by heating a stainless steel tube of 0.10 to 0.15 mm inner diameter. The resulting supersonic jet contains small droplets that vaporize futher due to the hot gas in this low-pressure region of the ion source. When an auxillary filament or low-current discharge device is used, complete evaporation of solvent from the liquid droplets produces gas phase ions from ionic compounds in the sample solution.

The particle beam (PB) interface, derived from MAGIC (Monodisperse Aerosol Generation Interface for Chromatography), has elements in common with thermospray, but gives spectra with many more fragment ions. Iron source is a beam of charged particles and formation of an aerosol is the initial step, followed by dispersion caused by a gas stream (usually helium), and desolvation. In the original interface, a momentum separator then separates the lighter dispersion gas and vaporized solvent from the sample particles having higher momentum, and finally ions enter the mass spectrometer. PB is less sensitive than thermospray or Electrospray and is not best suited for the analysis of ionic components, high molecular mass samples, and thermally labile compounds.

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