Surface Enhanced Laser, Desorption, Ionization, Biological Mixtures, Hydrophobic, Physiological Fluids, Purification

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis - Introduction Ionization Methods Matrix Assisted Laser Dersorption Ionization - MALDI Surface Enhanced Laser Desorption Ionization - SELDI Electrospray-ES Fast Atom Bombardment-FAB or Liquid Secondary Ionization-LSI Plasma Desorption Mass Spectrometry-PDMS Thermospray and Particle Beam-PB Electron Ionization-EI and Chemical Ionization-CI Mass Spectrometers and Mass Spectral Analysis Maldi TOF MS Linear TOF MS DE Maldi-TOF MS Reflectron TOF - RETOF-MS Quadrupole Mass Filter Instruments Trapping Instruments Tandem Mass Spectrometers Gas Chromatography Mass Spectrometry Liquid Chromatography and Tandem Mass Spectrometry LC-MS-MS

Home >> Biotechnology and Genomics >> Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis >>Surface Enhanced Laser Desorption-Ionization(SELDI)

Your Ad Here

Surface Enhanced Laser Desorption-Ionization (SELDI)

SELDI mass spectrometry is an ultra high throughput screening (uHTS) system for protein profiling of extracts obtained from the cells, tissue or physiological fluids. The patented SELDI protein chip system is a unique combination and miniaturization of technologies on a single, unified platform. This system enables capture, purification, analysis and processing of proteins from complex biological mixtures directly on protein chip array surface. Detection of the purified proteins is achieved by laser desorption/ionization time of flight mass spectrometric analysis. In this method, different selective surfaces such as cation or anion exchange surfaces, hydrophobic surfaces, metal binding surfaces, antibodies for specific proteins and DNA can be used on protein chip array to capture proteins and to study protein-protein interactions and protein-DNA interactions to identity proteins.

The SELDI process includes following steps: (i) The sample is applied on the selective surface of the protein chip, so that a subset of proteins is specifically bound to the protein chip. (ii) an energy absorbing molecular assembly (EMA) solution is added on the top of each spot to facilitate desorption and ionization (this solution works like matrix in MALDI). (iii) The chip is analysed by LDI-TOF-MS, which generates a protein spectrum of the different molecular masses present in the protein chip. (iv) The data is transferred to a specialized software package to facilitate data collection and further analysis.

This system can detect and accurately calculate the mases of compounds ranging from small molecules and peptides of less than 1 kDa to proteins of 500 kDa or more.

	Home
Isotope Ratio Mass Spectrometry - IRMS Applications of Mass Spectrometry for Genome Proteome Analysis DNA Sequencing by DE-MALDI-TOF MS SNP Detection Using MS Pinpoint Assay for SNP Detection Using MALDI-TOF MS of PCR Products SNP Detection Using PNA Probes SNP Detection Using Invader Cleavage SNP Detection Involving Minisequencing Using Maldi on a Chip Technology Proteome Analysis Using Mass Spectrometer Peptide Sequencing Determination of Molecular Weights of Intact Proteins Protein Identification by Combining Mass Spectrometer With Database Search Searching With Peptide Mass Fingerprints Searching With Tandem Mass Spectrometric Data Searching for Protein Modifications Analysis of Postranslational Modifications