SNP Detection, Mutations, Single Nucleotide Polymorphisms, Oligonucleotides, Ionization, Hybridization, Nucleic, Electrophoresis based

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Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis - Introduction Ionization Methods Matrix Assisted Laser Dersorption Ionization - MALDI Surface Enhanced Laser Desorption Ionization - SELDI Electrospray-ES Fast Atom Bombardment-FAB or Liquid Secondary Ionization-LSI Plasma Desorption Mass Spectrometry-PDMS Thermospray and Particle Beam-PB Electron Ionization-EI and Chemical Ionization-CI Mass Spectrometers and Mass Spectral Analysis Maldi TOF MS Linear TOF MS DE Maldi-TOF MS Reflectron TOF - RETOF-MS Quadrupole Mass Filter Instruments Trapping Instruments Tandem Mass Spectrometers Gas Chromatography Mass Spectrometry Liquid Chromatography and Tandem Mass Spectrometry LC-MS-MS

Home >> Biotechnology and Genomics >> Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis >>SNP Detection Using MS

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SNP Detection Using MS.
A variety of methods have been developed for the analysis of point mutations that are due to single nucleotide polymorphisms (SNP). Several methods that require neither the gel electrophoresis, nor labelling utilize the gel electrophoresis, nor labelling utilize MALDI-TOF MS. Study of DNA polymorphism using MS, depends largely on the determination of relative masses of individual DNA fragments with sufficient accuracy, so that even a single base replacement (transition or transversion) can be identified due to unique mass of each base. However, the methods have been applied only to relatively short oligonucleotides because the resolution and sensitivity of MALDI-MS generally falls off dramatically with increase in size of oligonucleotides.

Analysis of nucleic acid by MALDI-TOF MS has several advantages including the following: (1) The ionization Separation by size, and detection takes milliseconds to complete, so that the technology is fast; in contrast electrophoretic separation and detection takes hours, sometimes days. (ii) The results are more accurate than the electrophoresis-based and hybridization-based methods, which are influenced by secondary structures formed by nucleic acids. (iii) Complete automation involving both sample preparation and acquisition/processing of data is possible.

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Isotope Ratio Mass Spectrometry - IRMS Applications of Mass Spectrometry for Genome Proteome Analysis DNA Sequencing by DE-MALDI-TOF MS SNP Detection Using MS Pinpoint Assay for SNP Detection Using MALDI-TOF MS of PCR Products SNP Detection Using PNA Probes SNP Detection Using Invader Cleavage SNP Detection Involving Minisequencing Using Maldi on a Chip Technology Proteome Analysis Using Mass Spectrometer Peptide Sequencing Determination of Molecular Weights of Intact Proteins Protein Identification by Combining Mass Spectrometer With Database Search Searching With Peptide Mass Fingerprints Searching With Tandem Mass Spectrometric Data Searching for Protein Modifications Analysis of Postranslational Modifications