PCR Products SNP Detection, Gel Based Assays, Invasive Cleavage Assay, Oligonucleotide, Curr Science, Mutant Alleles

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis - Introduction Ionization Methods Matrix Assisted Laser Dersorption Ionization - MALDI Surface Enhanced Laser Desorption Ionization - SELDI Electrospray-ES Fast Atom Bombardment-FAB or Liquid Secondary Ionization-LSI Plasma Desorption Mass Spectrometry-PDMS Thermospray and Particle Beam-PB Electron Ionization-EI and Chemical Ionization-CI Mass Spectrometers and Mass Spectral Analysis Maldi TOF MS Linear TOF MS DE Maldi-TOF MS Reflectron TOF - RETOF-MS Quadrupole Mass Filter Instruments Trapping Instruments Tandem Mass Spectrometers Gas Chromatography Mass Spectrometry Liquid Chromatography and Tandem Mass Spectrometry LC-MS-MS

Home >> Biotechnology and Genomics >> Mass Spectrometry - An Essential Tool for Genome and Proteome Analysis >>SNP Detection Using Invader Cleavage

Your Ad Here

SNP Detection Using Invader Cleavage
Gel-based assays of PCR products for SNP detection. The presence of an SNP can be detected by RFLP or AFLP conducted on PCR products, whenever such an SNP generates or destroys a specific restriction site for an enzyme. SSCP can also be used for detection of SNPs. However, these methods for the detection of SNPs still need gel-based assays, and therefore, have recently been taken over by several other methods involving non- gel based assays.

Non-gel based assays of PCR products for SNP detection. Several non-gel based methods are avialable for SNP detection. If SNP is present at 3' end of a PCR primer binding site, it can be detected simply by the failure of amplification due to mismatch between the primer sequence and the binding site in the template, although it may be difficult to distinguish this failure of PCR due to SNP from a PCR failure due to other reasons.

Other non-gel based approaches, that are available for the detection of SNPs at an internal position of an amplicon generally need initial PCR amplification, followed by a non-gel based assay, that discriminates between wild and mutant alleles. There are still other assays available now, which neither need separation on the gel, nor do they need PCR amplification (e.g. 'invasive cleavage assay; for details. consult Gupta. 2001, Curr Science, Feb 26, 2001). The common non-gel based assays for detection of SNPs at the internal sites are based on the detection of mismatch between the PCR product and an oligonucleotide used as a probe.

	Home
Isotope Ratio Mass Spectrometry - IRMS Applications of Mass Spectrometry for Genome Proteome Analysis DNA Sequencing by DE-MALDI-TOF MS SNP Detection Using MS Pinpoint Assay for SNP Detection Using MALDI-TOF MS of PCR Products SNP Detection Using PNA Probes SNP Detection Using Invader Cleavage SNP Detection Involving Minisequencing Using Maldi on a Chip Technology Proteome Analysis Using Mass Spectrometer Peptide Sequencing Determination of Molecular Weights of Intact Proteins Protein Identification by Combining Mass Spectrometer With Database Search Searching With Peptide Mass Fingerprints Searching With Tandem Mass Spectrometric Data Searching for Protein Modifications Analysis of Postranslational Modifications