SNP Detection Involving Minisequencing Using ‘MALDI On A Chip’ Technology.
In this technology, nano-liter amount of sample from minisequencing SNP-analysis reactions are piezoelectrically pipetted onto a silicon chip. This silicon chip is directly inserted into mass spectrometer (MS) and each separate sample spot is automatically analysed using MALDI-TOF MS approach. High quality mass spectrum from 100 seprate spots (each containing only 8 femtomoles of an oligonucleotide) could be obtained successfully using this approach, within six minutes.