Proteome Analysis Using MS
In the post-genomic era, proteome analysis will be crucial for understanding different biological process. To achieve this objective, mass spectrometry has been and will be extensively utilized for
(i) peptide sequencing,
(ii) identification of proteins,
(iii) study of protein expression in different tissues and under different conditions,
(iv) identification of post-translation modification (phosphorylation, glycosylation, etc) of proteins in response to different stimuli and for
(v) characterization of protein interactions that include protein-ligand, protein-protein and protein-DNA interactions. Recent improvements in MS have led to its increased use for protein structure and function.