Use of Transposable Elements Transposon Tagging,Clone,Allele,Mutant,Antirrhinum Majus,Tagging,Genome,Snapdragon

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Isolation, Sequencing and Synthesis of Genes Isolation, Sequencing and Synthesis of Genes - Introduction Isolation of Genes Isolation of Genes Coding for Specific Proteins Isolation of Genes with Known or Unknown Products having Tissue Specific Expression Isolation of Genes with Known or Unknown Products Using DNA or RNA Probes Use of Heterologus Probes Use of Synthetic Probes Isolation of Genes Coding Use of Transposable Elements Transposon Tagging T-DNA-Insertion Mutagenesis for Isolation of Plant Genes Promoter Enhancer and Gene Trap for Isolation of Genes Differential Screeing and Differential Display Technique for Isolation of Genes Subtractive Hybridizatioon for Gene Isolation Map Based Cloning for Gene Isolation Isolation of Novel Genes by Asis Data at JNU New Delhi

Home >> Biotechnology and Genomics >> Isolation, Sequencing and Synthesis of Genes >> Use of Transposable Elements (Transposon Tagging)

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Use of Transposable Elements (Transposon Tagging).
The transposable elements (TEs), in some cases, have been effectively utilized for isolation of genes, when the gene product is unknown. In this case a transposon works as a gene tag. Following steps which are illustrated are involved in this procedure; (i) cone a known gene with a scorable phenotypic effect; (ii) TE is transposed to this gene to get an unstable allele; (iii) this unstable allele is cloned and TE is isolated from this Unstable allele (this is to select a TE which can produce unstable allele);

Use of transposon mutagenesis or tagging for identification and isolation of a gene

Use-of-transposon mutagenesis or Tagging For Identifiction and Isolation of a Gene

Genes isolated using transposon tagging in plants

Gene	Gene function	Species	Transposon (Tn)
A1	Enzyme required for anthocyanin biosynthesis	Maize	Mac En/Spm
B_z1	Enzyme required for anthocyanin biosynthesis	Maize	Ac
B_z2	Regulatory gene	Maize	Ds: Mu
C1	Enzyme required for anthocyanin biosynthesis	Maize	En/Spm
C2	Regulatory gene	Maize	En/Spm
P	Regulatory gene	Maize	Ac
R	Regulatory gene	Maize	Ac
V_pI	Leaf development	Maize	Mu
knotted	Regulatory gene	Maize	Ds
Opaque-2	Chloroplast development	Maize	Ac: En/Spm
Hef-106	Enzyme required for anthocyanin biosynthesis	Snapdragon	Mu

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(iv) This TE is transposed to a gene of interest with known phenotypic effect, to produce unstable allele; (v) the DNA is extracted from this mutant; (vi) TE sequence is used as a probe to isolate and clone the mutant gene (carrying inserted TE), so that we can then isolate the gene of interest.

In maize, TE like Ac/Ds En/Spm, and Mu/have been isolated using genes Wx, C2 and Adhi. Similarly, TEs like Tam3 and Tam7 have been isolated from snapdragon (Antirrhinum majus).

These TEs have been used for gene tagging experiments leading to isolation of genes. In maize, several genes like Bzl, P, Al, CI and C2 have also been isolated successfully using transposon tagging
For transposon tagging, often transposable elements endogenous to species like maize and snapdragon have been used. However, rarely transposons available from one plant species can be moved into the genome of another plant species, whose gene is to be isolated. For instance Ac element of maize has been transferred to tobacco, where it can integrate into any locus permitting transposon tagging and gene isolation.

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