Use of Synthetic Probes,Dimensional Gel,Electrophoresis,Amino Acids,Oligonucleotides,Automated DNA Synthesizers

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Isolation, Sequencing and Synthesis of Genes Isolation, Sequencing and Synthesis of Genes - Introduction Isolation of Genes Isolation of Genes Coding for Specific Proteins Isolation of Genes with Known or Unknown Products having Tissue Specific Expression Isolation of Genes with Known or Unknown Products Using DNA or RNA Probes Use of Heterologus Probes Use of Synthetic Probes Isolation of Genes Coding Use of Transposable Elements Transposon Tagging T-DNA-Insertion Mutagenesis for Isolation of Plant Genes Promoter Enhancer and Gene Trap for Isolation of Genes Differential Screeing and Differential Display Technique for Isolation of Genes Subtractive Hybridizatioon for Gene Isolation Map Based Cloning for Gene Isolation Isolation of Novel Genes by Asis Data at JNU New Delhi

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Use of synthetic probes.
If protein, purified using the technique of two dimensional gel electrophoresis, is used for microsequencing of 5-15 consecutive amino acids, this information can be used for the synthesis of oligonucleotides (using automated DNA synthesizers). These oligonucleotides may then be directly utilized for screening of cDNA or genomic libraries for isolation of specific genes (Fig. 8.3) These can also be used for designing primers for the amplification of a gene in extracted from a tissue. A 32 kilodalton (kD) glycoprotein (isolated from style) associated with the S₂ incompatibility allele of Nicotiana allata was used for synthesis of an oligonucleotide leading to the isolation of a related gene

Isolation of a gene using hte probe, artificiallysynthesized, on the basis of amino acid sequence of a part of the protein

Isolation of a Gene Using the Probe, Artificially Synthesized, on the Basis of Amino Acid Sequence of a Part of the Protein

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Sequencing of a Gene or a DNA Fragment Maxam and Gilbert's Chemical Degradation Method Sanger's Dideoxynucleotide Synthetic Method Automatic DNA Sequencers Slab Gel Versus Capillary Sequencers Slab Gel Sequencing Systems Capillary Gel Sequencing Systems Direct DNA Sequencing Using PCR Ligation Mediated PCR LMPCR DNA Sequencing Through Transcription DNA Sequencing by Hybridization Using Microarrays on DNA Chips DNA Sequencing by DE-MALDI-TOF Mass Spectrometry Synthesis of Genes Synthesis of Gene for Yeast Alanyl tRNA Synthesis of Oligonucleotides Synthesis of Three Duplex Fragments Synthesis of Gene from Three Duplex Fragments Synthesis of Gene for a True Precursor tRNA Total Synthesis of a Human Leukocyte Interferon Gene Gene Synthesis Machines Protection Chemistry and Amidites Permanent Protecting Groups Temporary Protecting Groups Synthesis of Gene Using PCR Synthesis of Genes From mRNA