T-DNA Insertion Mutagenesis for Isolation of Plant Genes,Transposon Tagging,Cross Fertilized Diploid Plants,Endogenous,Agrobacterium Tumefaciens

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Isolation, Sequencing and Synthesis of Genes Isolation, Sequencing and Synthesis of Genes - Introduction Isolation of Genes Isolation of Genes Coding for Specific Proteins Isolation of Genes with Known or Unknown Products having Tissue Specific Expression Isolation of Genes with Known or Unknown Products Using DNA or RNA Probes Use of Heterologus Probes Use of Synthetic Probes Isolation of Genes Coding Use of Transposable Elements Transposon Tagging T-DNA-Insertion Mutagenesis for Isolation of Plant Genes Promoter Enhancer and Gene Trap for Isolation of Genes Differential Screeing and Differential Display Technique for Isolation of Genes Subtractive Hybridizatioon for Gene Isolation Map Based Cloning for Gene Isolation Isolation of Novel Genes by Asis Data at JNU New Delhi

Home >> Biotechnology and Genomics >> Isolation, Sequencing and Synthesis of Genes >> T-DNA Insertion Mutagenesis for Isolation of Plant Genes

Your Ad Here

T-DNA Insertion Mutagenesis for Isolation of Plant Genes
A technique similar to transposon tagging is T-DNA insertion for isolation of genes. A limitation of transposon tagging is that it is often confined to either the self fertilized diploid plants such as snapdragon or the cross-fertilized diploid crops like maize, both having well characterized endogenous transposable elements (TE). Although derivatives of maize Ac have been successfully used as TE in crops to which they do not belong, their use is still limited and cannot be extended to all plant species. Therefore, T-DNA tagging involving random insertion of T-DNA into the genome, during Agrobacterium tumefaciens mediated transformation has been exploited.

Use of T-DNA insertion for identification and isolation of a gene

Use of T-DNA Insertion for Identification and Isolation of a Gene

The most extensive use of T-DNA insertion mutagenesis has been in Arbidopsis, where 35-40% mutations in cultures were found to be tagged by T-DNA inserts. In 1995, already there were as many as 14,000 Transformants, with an average of 1.5 inserts per transformant were available at the university of Arizona, Tucson (USA), 40% of them being due to T-DNA insertion. If Arabidopsis genome is considered to be 130 Mb and T-DNA insertion is random, there is 50% probability for any individual gene to be tagged among the above 14,000 transformants. A number of these genes have been cloned using T-DNA insertion and are being utilized for improvement of a variety of traits in a number of crop plants.

Your Ad Here

	Home
Sequencing of a Gene or a DNA Fragment Maxam and Gilbert's Chemical Degradation Method Sanger's Dideoxynucleotide Synthetic Method Automatic DNA Sequencers Slab Gel Versus Capillary Sequencers Slab Gel Sequencing Systems Capillary Gel Sequencing Systems Direct DNA Sequencing Using PCR Ligation Mediated PCR LMPCR DNA Sequencing Through Transcription DNA Sequencing by Hybridization Using Microarrays on DNA Chips DNA Sequencing by DE-MALDI-TOF Mass Spectrometry Synthesis of Genes Synthesis of Gene for Yeast Alanyl tRNA Synthesis of Oligonucleotides Synthesis of Three Duplex Fragments Synthesis of Gene from Three Duplex Fragments Synthesis of Gene for a True Precursor tRNA Total Synthesis of a Human Leukocyte Interferon Gene Gene Synthesis Machines Protection Chemistry and Amidites Permanent Protecting Groups Temporary Protecting Groups Synthesis of Gene Using PCR Synthesis of Genes From mRNA