Synthesis of Oligonucleotides,Pentanucleotide,Icosanucleotide,Phosphate Group,Hydroxyl Group,Nucleotide,Protective Groups

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Isolation, Sequencing and Synthesis of Genes Isolation, Sequencing and Synthesis of Genes - Introduction Isolation of Genes Isolation of Genes Coding for Specific Proteins Isolation of Genes with Known or Unknown Products having Tissue Specific Expression Isolation of Genes with Known or Unknown Products Using DNA or RNA Probes Use of Heterologus Probes Use of Synthetic Probes Isolation of Genes Coding Use of Transposable Elements Transposon Tagging T-DNA-Insertion Mutagenesis for Isolation of Plant Genes Promoter Enhancer and Gene Trap for Isolation of Genes Differential Screeing and Differential Display Technique for Isolation of Genes Subtractive Hybridizatioon for Gene Isolation Map Based Cloning for Gene Isolation Isolation of Novel Genes by Asis Data at JNU New Delhi

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Synthesis of Oligonucleotides
Fifteen oligonucleotides ranging from pentanucleotide (5 bases) to an icosanucleotide (20 bases) were synthesized. Synthesis was conducted through condensation between hydroxyl group at the 3' position of one nucleotide and phosphate group at 5' position of the second nucleotide. In order to bring about condensation, all other functional groups, not taking part in condensation were protected using specific protective groups. After protecting the groups, reaction between a nucleotide with protected 5' end and another nucleotide with protected 3' end proceed according to. Subsequently, condensation was also brought about between groups of two, three or four nucleotides.

Condensation between two nucleotides with protected 5' OH and 3' OH groups in the sugars and protected amino groups in the nitrogenous bases

Condensation Between Two Nucleotides With Protected 5' OH and 3' OH Groups in the Sugars and Protected Amino Groups in the Nitrogenous Bases

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Sequencing of a Gene or a DNA Fragment Maxam and Gilbert's Chemical Degradation Method Sanger's Dideoxynucleotide Synthetic Method Automatic DNA Sequencers Slab Gel Versus Capillary Sequencers Slab Gel Sequencing Systems Capillary Gel Sequencing Systems Direct DNA Sequencing Using PCR Ligation Mediated PCR LMPCR DNA Sequencing Through Transcription DNA Sequencing by Hybridization Using Microarrays on DNA Chips DNA Sequencing by DE-MALDI-TOF Mass Spectrometry Synthesis of Genes Synthesis of Gene for Yeast Alanyl tRNA Synthesis of Oligonucleotides Synthesis of Three Duplex Fragments Synthesis of Gene from Three Duplex Fragments Synthesis of Gene for a True Precursor tRNA Total Synthesis of a Human Leukocyte Interferon Gene Gene Synthesis Machines Protection Chemistry and Amidites Permanent Protecting Groups Temporary Protecting Groups Synthesis of Gene Using PCR Synthesis of Genes From mRNA