Slab Gel Sequencing Systems,Ultrathin,Automation,Capillary Action,Dideoxy Reaction,Plexiglass Block,Porous Combs

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Isolation, Sequencing and Synthesis of Genes Isolation, Sequencing and Synthesis of Genes - Introduction Isolation of Genes Isolation of Genes Coding for Specific Proteins Isolation of Genes with Known or Unknown Products having Tissue Specific Expression Isolation of Genes with Known or Unknown Products Using DNA or RNA Probes Use of Heterologus Probes Use of Synthetic Probes Isolation of Genes Coding Use of Transposable Elements Transposon Tagging T-DNA-Insertion Mutagenesis for Isolation of Plant Genes Promoter Enhancer and Gene Trap for Isolation of Genes Differential Screeing and Differential Display Technique for Isolation of Genes Subtractive Hybridizatioon for Gene Isolation Map Based Cloning for Gene Isolation Isolation of Novel Genes by Asis Data at JNU New Delhi

Home >> Biotechnology and Genomics >> Isolation, Sequencing and Synthesis of Genes >> Slab Gel Sequencing Systems

Slab Gel Sequencing Systems
These systems make use of ultrathin (75 μm) slab gels and involve running of atleast 96 lanes per gel, the runs being completed twice daily. In these systems, automation in sample loading of sequencing gels has also been achieved, by using 'a plexiglass block having wells that are same distance apart as the comb teeth cut in a porous membrane that is used as a comb for drawing samples by capillary action. Each well in plexiglass block is filled with a sample (PCR dideoxy-reaction mixture), so that when the porous membrane comb is lowered onto the sample wells in the plexiglass block, the samples are drawn up automatically into the comb teeth by capillary action.

Using this approach of employing porous combs, automated loading of upto 192, 384 or 480 samples per gel has been achieved. The porous comb with the samples is placed between the glass plates of the gel apparatus above the flat surface of the polymerized gel and the samples are driven from the comb into the gel by electrophoresis.

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Sequencing of a Gene or a DNA Fragment Maxam and Gilbert's Chemical Degradation Method Sanger's Dideoxynucleotide Synthetic Method Automatic DNA Sequencers Slab Gel Versus Capillary Sequencers Slab Gel Sequencing Systems Capillary Gel Sequencing Systems Direct DNA Sequencing Using PCR Ligation Mediated PCR LMPCR DNA Sequencing Through Transcription DNA Sequencing by Hybridization Using Microarrays on DNA Chips DNA Sequencing by DE-MALDI-TOF Mass Spectrometry Synthesis of Genes Synthesis of Gene for Yeast Alanyl tRNA Synthesis of Oligonucleotides Synthesis of Three Duplex Fragments Synthesis of Gene from Three Duplex Fragments Synthesis of Gene for a True Precursor tRNA Total Synthesis of a Human Leukocyte Interferon Gene Gene Synthesis Machines Protection Chemistry and Amidites Permanent Protecting Groups Temporary Protecting Groups Synthesis of Gene Using PCR Synthesis of Genes From mRNA

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