Permanent Protecting Groups,Amides,Benzoyl Group,Isobutyl Group,G.Thymine,Phosphite,Triester Phosphoramidite,Hydroxyl Group

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Isolation, Sequencing and Synthesis of Genes Isolation, Sequencing and Synthesis of Genes - Introduction Isolation of Genes Isolation of Genes Coding for Specific Proteins Isolation of Genes with Known or Unknown Products having Tissue Specific Expression Isolation of Genes with Known or Unknown Products Using DNA or RNA Probes Use of Heterologus Probes Use of Synthetic Probes Isolation of Genes Coding Use of Transposable Elements Transposon Tagging T-DNA-Insertion Mutagenesis for Isolation of Plant Genes Promoter Enhancer and Gene Trap for Isolation of Genes Differential Screeing and Differential Display Technique for Isolation of Genes Subtractive Hybridizatioon for Gene Isolation Map Based Cloning for Gene Isolation Isolation of Novel Genes by Asis Data at JNU New Delhi

Home >> Biotechnology and Genomics >> Isolation, Sequencing and Synthesis of Genes >> Permanent Protecting Groups

Your Ad Here	Permanent Protecting Groups These protecting groups are stable in all reaction steps and remain in position throughout the synthesis. They are removed only after the synthesis is completed. Primary amino groups on the heterocyclic bases are generally protected as amides, using benzoyl group for A or C, and isobutyl group for G. Thymine has no amino group and thus needs no protection. Deprotection is done by using concentrated ammonia at 550C overnight (~12 hours). However, if PAC (phenoxyacetyl) amidite protection strategy is used, the deprotection time can be reduced to I hour at 70.C.
Protection of primary amino groups on heterocyclic bases as amides (amino groups in adenine and cytosine are protected using benzoyl group and amino group in guanine is protected using isobutyl group
Structures of pheoxyacetyl (PAC) and Isobutyl (ibu) group used in PAC amidite protection strategy Protection of -NH₂ group by PAC and phosphate group by B cyanoethyl group to produce an amidite	Your Ad Here
For protection of phosphate group in gene synthesizers, phosphite triester (phosphoramidite) chemistry is used. In this method, hydroxyl group on the phosphorus atom is protected using a methyl or β-cyanoethyl group, the latter is removed by treatment with concentrated ammonia after the synthesis is completed. This also means that the protecting groups in the bases as well as in the phosphate can be deprotected in one step using ammonia.

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