Maxam and Gilbert's Chemical Degradation Method,Reagent,Electrophorese,Fragments Autoradiograph,Lanes Gel

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Home >> Biotechnology and Genomics >> Isolation, Sequencing and Synthesis of Genes >> Maxam and Gilbert's Chemical Degradation Method

Maxam and Gilbert's Chemical Degradation Method
In this method, which is illustrated in Figure 8.11, following steps are involved:
(i) Label the 3' end of DNA with 32p.
(ii) Separate two strands, both labelled at 3' ends.
(iii) Divide the mixture in four samples, each treated with a different reagent having the property of destroying either only G, or only C, or G and A or C and T. The concentration of reagent is so adjusted that 50% of target base is destroyed, so that fragments of different sizes having 32p are produced.
(iv) Electrophorese each of the four samples in four different lanes of the gel.

Different Steps Involved in Maxam and Gilberts Method of DNA Sequencing

Different steps involved in Maxam and Gilberts method of DNA sequencing

An Autiograph of a Maxam and Gilberts sequencing gel

An autoradiograph of a Maxam And gilbeerts Sequencing Gel the Sequence Read From the Gel is shown To the Left

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