Isolation of Novel Genes by Asis Datta at JNU, New Delhi,Gene for Oxalate Decarboxylase,Datta Laboratory,Optimum,Lysine,Amaranthus

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Isolation, Sequencing and Synthesis of Genes Isolation, Sequencing and Synthesis of Genes - Introduction Isolation of Genes Isolation of Genes Coding for Specific Proteins Isolation of Genes with Known or Unknown Products having Tissue Specific Expression Isolation of Genes with Known or Unknown Products Using DNA or RNA Probes Use of Heterologus Probes Use of Synthetic Probes Isolation of Genes Coding Use of Transposable Elements Transposon Tagging T-DNA-Insertion Mutagenesis for Isolation of Plant Genes Promoter Enhancer and Gene Trap for Isolation of Genes Differential Screeing and Differential Display Technique for Isolation of Genes Subtractive Hybridizatioon for Gene Isolation Map Based Cloning for Gene Isolation Isolation of Novel Genes by Asis Data at JNU New Delhi

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Your Ad Here	Isolation of Novel Genes by Asis Datta at JNU, New Delhi In the year 1992, Prof. Asis Datta of JNU, New Delhi was selected for the Birla A ward for Science and Technology for cloning and characterization of the following two novel genes, relevant to human health. Gene for oxalate decarboxylase. In 1991, Prof. Datta's laboratory reported for the first time the cloning and characterization of the oxalate decarboxylase gene, which degrades and will thus reduce in our crop produce the content of oxalate or oxalic acid, which is harmful in many ways including the following: (i) Several green leafy vegetables (e.g. Amaranthus, spinach, etc.) being rich in oxalate, if ingested in large quantity are toxic, since oxalate chelates calcium and also destroys renal tissue in kidney. (ii) The attack and spread of a fungus (Whetzelinia sclerotium), which causes damage to crops like sunflower involves accumulation of oxalic acid in the infested tissue.
(iii) Oxalic acid is an essential substrate for the synthesis of the neurotoxin called β-N-oxalyl I-L-α, β Diamino-propionic acid (ODAP), found in different parts of the plants of Lathyrus sativus (khesari dal). Consumption of this legume causes neurolathyrism, a well known disease causing damage to leg muscles, etc. ODAP interferes with metabolism of glutamic acid, which is involved in transmission of nerve impulses in the brain, so that despite its rich protein content, L. sativus can not be used as a food source. In all the above cases (leafy vegetables, sunflower and L sativus), transfer of single gene for oxalate decarboxylase to produce transgenic plants will improve these plant species by reducing the oxalate content. This new gene will also allow the development of a 'diagnostic kit to measure oxalate in blood and urine’, since prevalent methods for clinical tests are quite expensive.
Gene for a seed specific protein with nutritionally balanced composition of amino acids. Prof. Datta's laboratory also reported. for the first time (in 1992) isolation of a gene from Amaranthus that encodes a seed specific protein rich in all essential amino acids including lysine and sulphur rich amino acids. Interestingly, the amino acid composition of this protein corresponds to that of WHO recommended protein standard for the optimum human nutrition. It is known that seed proteins in cereals as well as in legumes (peas, etc.) are deficient, the cereal proteins being deficient in lysine and pulses being deficient in sulphur containing amino acids. In view of this, the gene isolated from Amaranthus will be utilized in future for compensating the amino acid deficiencies of many seed proteins, once it is genetically engineered into the target crop plants. Efforts have been made jointly by Prof. Asis Datta and the Department of Biotechnology, Govt. of India, to seek patent rights for this gene in USA and Europe. The gene has also been successfully used for production of transgenic potato.	Your Ad Here

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