Capillary Gel Sequencing System,Slab Gel,DNA Fragments,Reloaded,Adenine,Cytosine,Guanine,Thymine,MegaBACE,Migrates,Capillary Tube

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Isolation, Sequencing and Synthesis of Genes Isolation, Sequencing and Synthesis of Genes - Introduction Isolation of Genes Isolation of Genes Coding for Specific Proteins Isolation of Genes with Known or Unknown Products having Tissue Specific Expression Isolation of Genes with Known or Unknown Products Using DNA or RNA Probes Use of Heterologus Probes Use of Synthetic Probes Isolation of Genes Coding Use of Transposable Elements Transposon Tagging T-DNA-Insertion Mutagenesis for Isolation of Plant Genes Promoter Enhancer and Gene Trap for Isolation of Genes Differential Screeing and Differential Display Technique for Isolation of Genes Subtractive Hybridizatioon for Gene Isolation Map Based Cloning for Gene Isolation Isolation of Novel Genes by Asis Data at JNU New Delhi

Home >> Biotechnology and Genomics >> Isolation, Sequencing and Synthesis of Genes >> Capillary Gel Sequencing System

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Capillary Gel Sequencing Systems
In these systems, slab gel electrophoresis is replaced by capillary gel electrophoresis to analyse DNA samples. In these systems, instead of scanning DNA as it migrates through 96 lanes each in a series of poured slab gels, DNA is sent through a set of 96 capillary tubes filled with a polymerised gel.

As the DNA fragments pass through these capillary tubes, DNA fragments are scanned. In the original models of the above old slab gel machines, gels must-be poured and reagents frequently reloaded, interrupting the sequencing. In capillary gel sequencing systems, on the other hand, the robot moves the DNA samples and reagents through the tubes continuously, requiring attention only once a day. The system produces a steady flow of data, each signal representing one of the four DNA bases (adenine, cytosine, guanine and thymine).

More recently (in 2002), 'Amersham Pharmacia Biotech' has marketed its improved model MegaBACE 4000, which makes use of 384 capillaries thus increasing many-fold the speed of sequencing. As mentioned above, due to competition with these capillary sequencing systems, automation has-now been achieved in several models of slab gel systems also.

-Genome DNA - Cloned DNA	DNA Amplification (PCR)	Sequencing reactions	Automated analysis of sequencing reactions
1.Mutation screening for known genes	1. Generation of sequencing templates from genomic DNA using PCR (ligation of adapters of known sequences for designing primers)	1. Annealing of sequencing primer.	1. Electrophoretic separation of sequencing reactions.
2. Isolation of DNA fragments by cloning	2. Generation of sequencing templates from cloned fragements using PCR and primers to vector sites flanking the insert.	2. Labelling reaction.	2. Computer registration of sequencing data.
		3. Termination reaction.
		4. Stop.

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