Automatic DNA Sequencers Slab-Gel Versus Capillary Sequencers,Perkin Elmers,Genetic Analyser,Amersham Pharmacia Biotech MegaBACE

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Isolation, Sequencing and Synthesis of Genes Isolation, Sequencing and Synthesis of Genes - Introduction Isolation of Genes Isolation of Genes Coding for Specific Proteins Isolation of Genes with Known or Unknown Products having Tissue Specific Expression Isolation of Genes with Known or Unknown Products Using DNA or RNA Probes Use of Heterologus Probes Use of Synthetic Probes Isolation of Genes Coding Use of Transposable Elements Transposon Tagging T-DNA-Insertion Mutagenesis for Isolation of Plant Genes Promoter Enhancer and Gene Trap for Isolation of Genes Differential Screeing and Differential Display Technique for Isolation of Genes Subtractive Hybridizatioon for Gene Isolation Map Based Cloning for Gene Isolation Isolation of Novel Genes by Asis Data at JNU New Delhi

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Automatic DNA Sequencers (Slab-Gel Versus Capillary Sequencers)
A variant of the above dideoxy-method was later developed, which allowed the production of automatic sequencers (.e.g. Perkin Elmer's ABI Prism 310 or 3700 Genetic Analyser; Amersham Pharmacia Biotech's MegaBACE 1000 and 4000).

In this new approach,' different fluorescent dyes are tagged either to the oligonucleotide primer (dye primers) in each of the four reaction tubes (blue for A, red for C, etc.), or to each of the four ddNTPs (dye terminators) used in a single reaction tube; when four tubes are used, they are pooled. After the PCR reaction is over, the reaction mixture is subjected to separation of synthesized fragments through electrophoresis. Depending upon the electrophretic system used, whether slab gel electrophoresis or capillary electrophoresis, following two types of automatic sequencing systems have been designed.

Fluorescene detection of oligonucleotide fragments produced by Sangers dideoxy method

Fluorescence detection of Oligonucleotide Fragments Produced by Sangers Dideoxy Method

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Sequencing of a Gene or a DNA Fragment Maxam and Gilbert's Chemical Degradation Method Sanger's Dideoxynucleotide Synthetic Method Automatic DNA Sequencers Slab Gel Versus Capillary Sequencers Slab Gel Sequencing Systems Capillary Gel Sequencing Systems Direct DNA Sequencing Using PCR Ligation Mediated PCR LMPCR DNA Sequencing Through Transcription DNA Sequencing by Hybridization Using Microarrays on DNA Chips DNA Sequencing by DE-MALDI-TOF Mass Spectrometry Synthesis of Genes Synthesis of Gene for Yeast Alanyl tRNA Synthesis of Oligonucleotides Synthesis of Three Duplex Fragments Synthesis of Gene from Three Duplex Fragments Synthesis of Gene for a True Precursor tRNA Total Synthesis of a Human Leukocyte Interferon Gene Gene Synthesis Machines Protection Chemistry and Amidites Permanent Protecting Groups Temporary Protecting Groups Synthesis of Gene Using PCR Synthesis of Genes From mRNA