Single Strand Conformation Polymorphism SSCP, Technology allows Detection, Internal Site, Sensitivity Detect Polymorphism, Complete Sequencing Excels

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Single strand conformation polymorphism (SSCP).

This technology allows detection of polymorphism due to differences of one or more base pairs in the PCR products.

The technique relies on the secondary structure being different for single strands derived from PCR products that differ by one or more nucleotides at an internal site within the strand.

In order to detect such differences, PCR products are denatured and electrophoretically separated in neutral acrylamide gels.

PCR products that do not differ in fragment length have been shown to exhibit SSCP in several studies.

Since only the PCR products are subjected to SSCP, sequence information is often needed for designing the PCR primers, where isotopic labelling and autoradiography are used to detect the SSCP variants.

This makes the technique relatively unsuitable for routine mapping/tagging studies, although in its sensitivity to detect polymorphism, only complete sequencing excels it.

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Amplifed Fragment Length Polymorphism (AFLP) Single Strand Conformation Polymorphism (SSCP) Molecular Markers based on PCR Followed by Hybridization Oligonucleotide Fingerprinting Using RAPD/MP-PCR Framents Sequence based Molecular Markers Single Nucleotide Polymorphisms (SNPs) Gel-based Assays of PCR Products for SNP Detection Non-Gel based Assays of PCR Products for SNP Detection Taqman Assay Use of Molecular Beacon Oligonucleotide Ligation Assay (OLA) DNA Chip/Microarray Dynamic Allele-specific Hybridization Minisequencing Pyrosequencing for SNP Genotyping Temperature Modulated Heteroduplex Analysis TMHA Using DHPLC Wave^TM System A Non PCR Invasive Clevage Assay and Maldi TOF-MS for SNP Detection Comparison of Different Types of Molecular Markers High Throughput Approach in Molecular Marker Technology Comparison of General Features of Different types of Molecular and their use