Randm Amplified Microsatellite Retroposon Micro Satellite Amplified Polymorphism, Anchored Microsatellite, Copia Specific, Retroposon Isolate, Amplified Products

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DNA-Based Molecular Markers in Biotechnology DNA-Based Molecular Markers in Biotechnology Introduction Types of Molecular Markers/Principles Methods and Uses Hybridization Based Molecular Markers Restridization Fragment Length Polymorphisms (RFLPs) List Acronyms for Different DNA Markers along with References Dispersed Repetitive DNA (drDNA) for DNA Fingerprinting PCR based Molecular Markers PCR Methods Using a Single Primer PCR Methods Using a Pair of Primers Sequence-tagged Sites (STSs) and Sequence Characterized Amplified Regions (SCARs) Simple Sequence Repeats (SSRs) Expressed Sequence tags (ESTs) and their use for STSs,SSRs and SNPs Important Plant Genomes Available in dbEST Random Amplified Microsatellite Polymorphism (RAMP) and Retroposon-Micro-Satellite Amplified Polymorphism (REMAP)

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Random amplified microsatellite polymorphism (RAMP) and retroposon-microsatellite amplified polymorphism (REMAP).

In this approach, amplification is performed using a pair of primers, of which one primer is an anchored microsatellite (SSR) and the other primer can be either a RAPD primer (as in RAMP) or a retroposon L TR primer (as in REMAP.

In a study, in barely, while using REMAP, Copia-specific BARE-I primer (BARE-1 retroposon was isolated from barely) was used with an anchored SSR primer, so that the acronym copia-SSR was used for this technique.

In both these approaches (RAMP and REMAP), the amplified products resolve length polymorphism that may be present either at the SSR target site itself, or at the associated sequence between the binding sites of the two primers.

The RAPD/retroposon primer binding site actually serves as an arbitrary endpoint for the SSR-based specific, amplified sequence, as its is with ISAIISSR, where a single anchored primer is used.

The amplified products may also be digested with a restriction enzyme, to resolve further the polymorphism, so that it is then described as 'digested RAMPs (dRAMPs)' as done in barley.

The merit of RAMP and REMAP lies in the fact that undigested total genomic DNA is used as a template, instead of preamplified restriction digested DNA that is used in AFLP/SAMPLIS-SAP (to be discussed in the next section).

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Amplifed Fragment Length Polymorphism (AFLP) Single Strand Conformation Polymorphism (SSCP) Molecular Markers based on PCR Followed by Hybridization Oligonucleotide Fingerprinting Using RAPD/MP-PCR Framents Sequence based Molecular Markers Single Nucleotide Polymorphisms (SNPs) Gel-based Assays of PCR Products for SNP Detection Non-Gel based Assays of PCR Products for SNP Detection Taqman Assay Use of Molecular Beacon Oligonucleotide Ligation Assay (OLA) DNA Chip/Microarray Dynamic Allele-specific Hybridization Minisequencing Pyrosequencing for SNP Genotyping Temperature Modulated Heteroduplex Analysis TMHA Using DHPLC Wave^TM System A Non PCR Invasive Clevage Assay and Maldi TOF-MS for SNP Detection Comparison of Different Types of Molecular Markers High Throughput Approach in Molecular Marker Technology Comparison of General Features of Different types of Molecular and their use