Oligonucleotide Ligation Assay OLA, Nonel Approach, Biotinylated, Probes Anneal, Undergo Ligation Resulting, Streptavidin Coated, Matrix and Signal Detected

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Home >> Biotechnology and Genomics >> DNA-Based Molecular Markers in Biotechnology >>Oligonucleotide Ligation Assay OLA

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Oligonucleotide ligation assay (OLA).

This is a nonel approach, where two independent probes (one is 5' biotinylated and the other 3' labelled) are used for hybridization with PCR product,

so that, when the probes match the product, the two probes anneal with the PCR product and undergo ligation resulting in an oligonucleotide which is biotinylated at 5' end and labelled at 3' end.

The ligation product, which is labelled at 3'end, is captured on a solid streptavidin-coated matrix and signal is detected by autoradiography.

However, when there is a mismatch due to the presence of an SNP, labelled and biotinylated oligonucleotides are unable to ligate,

so that when captured by streptavidin, they carry no signal.

Technique of Oligonucleotide Ligation Assay

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