DNA Based Molecular Markers in Biotechnology Introduction, Amplification of Small DNA Fragments, Broad Area Biotechnology, Polymorphism Detected

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DNA-Based Molecular Markers in Biotechnology DNA-Based Molecular Markers in Biotechnology Introduction Types of Molecular Markers/Principles Methods and Uses Hybridization Based Molecular Markers Restridization Fragment Length Polymorphisms (RFLPs) List Acronyms for Different DNA Markers along with References Dispersed Repetitive DNA (drDNA) for DNA Fingerprinting PCR based Molecular Markers PCR Methods Using a Single Primer PCR Methods Using a Pair of Primers Sequence-tagged Sites (STSs) and Sequence Characterized Amplified Regions (SCARs) Simple Sequence Repeats (SSRs) Expressed Sequence tags (ESTs) and their use for STSs,SSRs and SNPs Important Plant Genomes Available in dbEST Random Amplified Microsatellite Polymorphism (RAMP) and Retroposon-Micro-Satellite Amplified Polymorphism (REMAP)

Home >> Biotechnology and Genomics >> DNA-Based Molecular Markers in Biotechnology >>DNA Based Molecular Markers in Biotechnology Introduction

DNA-Based Molecular Markers in Biotechnology

The techniques of cloning and PCR amplification of small DNA fragments to obtain millions of copies of each such fragment.

These techniques are now extensively used for a variety of purposes in the broad area of biotechnology.

One of the major uses of these tools is the development and use of DNA-based molecular markers for a variety of purposes.

These molecular markers are based on the polymorphism detected at the level of small DNA fragments.

During a period of two decades spanning over 1980 to 2000, a broad range of molecular markers became available, which are being used in a variety of ways.

A large number of acronyms for these molecular markers are in common usage now.

The major uses of these molecular markers is the development of molecular maps, that will be described in for humans and animal systems and for a variety of plant systems.

Only the basic principles and methods involved in the development and uses of these molecular markers will be discussed, since they are common to all kinds of organisms.

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Amplifed Fragment Length Polymorphism (AFLP) Single Strand Conformation Polymorphism (SSCP) Molecular Markers based on PCR Followed by Hybridization Oligonucleotide Fingerprinting Using RAPD/MP-PCR Framents Sequence based Molecular Markers Single Nucleotide Polymorphisms (SNPs) Gel-based Assays of PCR Products for SNP Detection Non-Gel based Assays of PCR Products for SNP Detection Taqman Assay Use of Molecular Beacon Oligonucleotide Ligation Assay (OLA) DNA Chip/Microarray Dynamic Allele-specific Hybridization Minisequencing Pyrosequencing for SNP Genotyping Temperature Modulated Heteroduplex Analysis TMHA Using DHPLC Wave^TM System A Non PCR Invasive Clevage Assay and Maldi TOF-MS for SNP Detection Comparison of Different Types of Molecular Markers High Throughput Approach in Molecular Marker Technology Comparison of General Features of Different types of Molecular and their use

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