Comparison of Different Types of Molecular Markers, Molecular Markers, Automation Approaches, Reproducible, Characterization of Genomes

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Your Ad Here	Comparison of Different Types of Molecular Markers In order to make a choice from a number of molecular markers that have now become available, it is necessary to make a comparison between different types of molecular markers (e.g. RFLPs, RAPDs, SSRs, AFLPs, SNPs, etc.) described in this chapter. These markers have often been compared for convenience, reproducibility, speed of assay, cost effectiveness and feasibility of using automation and high throughput approaches. For instance, in many studies, RAPDs were not always reproducible, while AFLP and SSR gave high reproducibility.
As mentioned above, the SSR markers are locus specific and co-dominant, thus making them the markers of choice. However, if SSR markers are developed for the first time, a very high cost of their development (due to cloning and sequencing) will restrict their use in many laboratories. But if SSR primers have already been developed in a species, the use of SSR markers for this species will be cost effective and can be used even in small laboratories (e.g. in wheat, -1000 primer pairs are already available . Polymorphic bands in RAPD, AFLP and SAMPL, can also be converted into locus specific SCARs, which will then prove to be as effective as SSR markers.
However, for characterization of genomes (fingerprinting) in a population, in our opinion, ISSRs, oligonucleotide fingerprinting, AFLPs and SAMPL may be more suitable, since each of them yields a large number of bands.	Your Ad Here