A non-PCR invasive cleavage assay, and MALDI-TOF MS for SNP detection.
In all the methods of SNP analysis described above, an initial target amplification using PCR is needed.

However, PCR does have significant limitations, when used in a high throughput approach, so that approaches involving simpler and more direct analysis of DNA without prior PCR amplification have been developed.

One such novel non-PCR approach employs "invasive cleavage assay" and a MALDI-TOF-MS detection system (for details, see Curr Sci, Feb. 26, 2001). etc.)