Ink Jet Printing Technology, Phospharamidities, Hydrophobic material, Oxidize, Protogene, Incyte, Hewlett Packard, Rosetta

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DNA Chip Technology and Microarrays DNA Chip Technology and Microarrays - Introduction Types of DNA Chips and Their Production Production of Oligonucleotide Microarrays Light Directed Deprotection Method Ink Jet Printing Technology Production of cDNA Microarrays

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Ink-jet printing technology.
Oligonucleotide micro arrays can also be produced by ink-jet printing technology, which involves direct delivery of phosphoramidites, rather than the use of photolithographic masks.
Following steps are involved:
(i) glass surface is coated with a light sensitive hydrophobic material,
(ii) synthetic areas are prepared by photolithographic etching,
(iii) phosphoramidites are delivered at specific hydrophilic wells created as above, and deblocking agent is used to oxidize and complete one round of coupling.

The cycle is repeated by delivering specific phosphoramidites at specific addresses. This ink-jetting is simpler, so that it may allow oligouncleotide micro arrays to reach a large number of researchers. However, only modest densities (10,000 features/cm²) are attainable by this technology. Microarrays using this technology are being commercialized by Protogene, lncyte, Hewlett Packard, Rosetta and several other companies.

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