Phagemid pBlueScript IIKS, Biology Laboratories, Orientation of Polylinker, Generate Single Stranded DNA

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Cloning and Expression Vectors Cloning and Expression Vectors Introduction Cloning Vectors for Recombinant DNA Plasmids as Vectors Three Phases of the Development of Plasmid Cloning Vectors Alpha-Complementation for Screening Using "blue-white"Clonies Nonsense Suppressors to Complement Termination Codons in Vectors E.Coli Plasmid Vectors pBR322 and pBr327 Vectors pUC Vectors pSP64-pSP65 pGEM-3, pGEM-4, pGEM-3Z, pGEM-4Z ΠAN13 from ΠAN7 Yeast Plasmid Vectors Retriever Vectors Agrobacterium Plasmid Vectors Lambda Phage (λ)Vectors Lambda Phage (Lysis and Lysogeny) Genetic Markers for the Selection and Screening of λ Vectors Development of Lambda (λ)Phage Vectors Insertion λ Vectors Replacement λ Vectors M13 Phage as a Vector for DNA Sequencing M13-vectors-of-mp-Series Hosts for M13 Vectors Cosmids as Vectors Phagemids as Vectors Phagemids pUC118 and pUC119 Phagemid pBluesript IIKS

Home >> Biotechnology and Genomics >> Cloning and Expression Vectors >> Phagemid pBluescript IIKS

Your Ad Here

Phagemid pBluescript IIKS

pBluescript II KS, is the most commonly used vector in molecular biology laboratories world over. It is derived from pUC19 and is 2961 bp (bp = base pairs) long. The KS designation indicates the orientation of poly linker, such that the transcription of lacZ gene proceeds from the restriction site for KpnI to that for Sad.

It is also described as pBluescript M13 +/-, because it carries M13 origin of replication in opposite orientations to generate single stranded DNA in vivo or RNA in vitro. It has been derived from pUC plasmid that contains promoters from T3 and T7 phages in opposite orientations.

Phagemid pBluescript IIKS

Phagemid pBluescript IIKS +/- and it major characteristics

The detailed structure of pBluescript II KS (+/-) is given in. It may be noted that it has the following features: (i) a multiple cloning site (MCS) flanked by T₃ and T₇ promoters to be read in opposite directions on the two strands, (ii) an inducible lac promoter (lad), upstream of lacZ region, which complements with E. coli (lacZ^-) and provides the facility for selection of chimeric vector DNA (recombinant vector) using the criterion of white colonies (as against blue colonies obtained if no foreign DNA is inserted)

(iii) f1 (+) and f1 (-) origins of replication derived from a filamentous phage (M13 phage) for recovery of sense single stranded DNA i.e. (+) and antisense (-) strands of lacZ gene, when host is coinfected with a helper phage; (iv) an origin of replication (Co1El ori) derived from plasmid, and used in the absence of helper phage; (iv) a gene for ampicillin resistance for antibiotic for selection of chimeric phage mid vector.

Your Ad Here

	Home
BACs and PACs as Vectors for Cloning large DNA Segments (100-300 kb) Bacterial Artificial Chromosomes (BACs) P1-derived Artificial Chromosomes (PACs) Plant and Animal viruses as Vectors Plant Viruses (Cauliflower Mosaic virus or CaMV and Geminiviruses) Animal Viruses Transposons as Vectors Transposons of Higher Plants (Ac/Dc or Mul of Maize) Transposons of drosophila (P elements) YAC and MAC Vectors for Cloning very large DNA Segments (1000 kb) Yeast Artificial Chromosomes (YACs) Mammalian Artificial chromosomes (MACs) Expression Vectors Promoters Nopaline Synthase (nos) Promoter from T-DNA Dual Promoter of Mannopine Synthase (mas) genes 1 and 2 35S Promoter of CaMV Polyhedrin Promoter from Baculovirus Expression Cassettes Baculovirus and Expression Vector System for Insect Cells Virus Expression Vectors for Mammalian Cells Binary and Shuttle Vectors