Nonsense Suppressors to Complement Termination Codons in Vectors, Phage Lambda, Nonsense Mutations, Essential Genes, Specific Strains

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Nonsense suppressors to complement termination codons in vectors.

Some vectors (e.g., phage lambda, Charon 4A and Charon 21A) used in recombinant DNA research contain nonsense mutations in essential genes. These vectors can not be propagated in all strains of E. coli, but only in some specific strains containing nonsense suppressor genes, so that the defective genes in the vectors are translated despite the 'presence of nonsense mutations (stop codons).

For instance charon 4A and charon 21A need, but charon 32-35 and charon 40 do not need suppressor gene in the host. The nonsense suppressor genes, as we know are tRNA genes, which can read the UAG (amber codon) or UAA (ochre codon). Rarely UGA (opal codon) is also similarly utilized. A number of such nonsense suppressors (supl, sup2, sup3, supE, supF) have been used in developing E. coli strains that complement vectors carrying genes with nonsense mutations.

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