M13 Vectors of mp Series, Intergenic Regions, Foreign DNA, Series of Vectors, Regulatory Sequences, Amino Acids

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Cloning and Expression Vectors Cloning and Expression Vectors Introduction Cloning Vectors for Recombinant DNA Plasmids as Vectors Three Phases of the Development of Plasmid Cloning Vectors Alpha-Complementation for Screening Using "blue-white"Clonies Nonsense Suppressors to Complement Termination Codons in Vectors E.Coli Plasmid Vectors pBR322 and pBr327 Vectors pUC Vectors pSP64-pSP65 pGEM-3, pGEM-4, pGEM-3Z, pGEM-4Z ΠAN13 from ΠAN7 Yeast Plasmid Vectors Retriever Vectors Agrobacterium Plasmid Vectors Lambda Phage (λ)Vectors Lambda Phage (Lysis and Lysogeny) Genetic Markers for the Selection and Screening of λ Vectors Development of Lambda (λ)Phage Vectors Insertion λ Vectors Replacement λ Vectors M13 Phage as a Vector for DNA Sequencing M13-vectors-of-mp-Series Hosts for M13 Vectors Cosmids as Vectors Phagemids as Vectors Phagemids pUC118 and pUC119 Phagemid pBluesript IIKS

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Your Ad Here	M13 vectors of mp series. As described earlier, the M13 genome contains two intergenic regions (one between genes II and IV, the other between genes III and VIII). M 13 mp series of vectors make use of intergenic region between the genes II and IV for insertion of foreign DNA. The other smaller intergenic region between the genes III and VIII has been used as the cloning site in another series of vectors, that are less frequently used.
The mp series vectors of M13 were derived from the phage M13mpl, which carries in its major IG region a DNA segment (from E. coli) containing some regulatory sequences and a part of lacZ gene encoding the first 146 amino acids (α-peptide) of galactosidase enzyme. In presence of IPTG and X-gal, this allows screening of chimeric phage particles carrying foreign DNA due to blue/white colonies, when grown on E. coli strains with a defective galactosidase gene (lacking sequence encoding 11-41 amino acids). This is called α-complementation as earlier described. Although Ml3 vectors with antibiotic resistance as the selectable markers were also designed, they are not commonly used.
Therefore all cloning of single stranded DNA is currently carried out with Ml3mp18 and M13mpl9 which carry 13 cloning sites (M13mp18 and Ml3mp19 carry these sites in opposite orientation) for accepting foreign DNA. Due to opposite orientations of cloning sites in M 13mp 18 and M13mp19, the progeny of one of these vectors will contain one strand of foreign DNA and the progeny of the second vector will contain the complementary strand automatically. Consequently, by using M13mp18 and M13mp19 as dual vectors, it is possible to use single primer (universal primer) to determine the sequence of nucleotides on opposite strands from each end of the inserted DNA. The polycloning sites and their orientations, in M13mpl8 and M13mpl9 are the same as those in pUC18 and pUC19, so that the fragments of foreign DNA can be moved between M13mp vectors and pUC vectors with great ease. Other similar pairs of vectors of mp series are MI3mp8 and M13mp9; MI3mpl2 and M 13mp 13. They correspond to similarly numbered plasmid vectors of pUC series. (pUC8 and pUC9; pUC12 and pUC13), particularly with respect to polylinker cloning site.	Your Ad Here

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