Cloning and Expression Vectors Introduction, Recombinant DNA, Genetic Engineering, DNA Segment, Plasmids and Phages Reproduce

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Cloning and Expression Vectors Cloning and Expression Vectors Introduction Cloning Vectors for Recombinant DNA Plasmids as Vectors Three Phases of the Development of Plasmid Cloning Vectors Alpha-Complementation for Screening Using "blue-white"Clonies Nonsense Suppressors to Complement Termination Codons in Vectors E.Coli Plasmid Vectors pBR322 and pBr327 Vectors pUC Vectors pSP64-pSP65 pGEM-3, pGEM-4, pGEM-3Z, pGEM-4Z ΠAN13 from ΠAN7 Yeast Plasmid Vectors Retriever Vectors Agrobacterium Plasmid Vectors Lambda Phage (λ)Vectors Lambda Phage (Lysis and Lysogeny) Genetic Markers for the Selection and Screening of λ Vectors Development of Lambda (λ)Phage Vectors Insertion λ Vectors Replacement λ Vectors M13 Phage as a Vector for DNA Sequencing M13-vectors-of-mp-Series Hosts for M13 Vectors Cosmids as Vectors Phagemids as Vectors Phagemids pUC118 and pUC119 Phagemid pBluesript IIKS

Home >> Biotechnology and Genomics >> Cloning and Expression Vectors >> Cloning and Expression Vectors Introduction

Your Ad Here

Cloning and Expression Vectors

Techniques for manipulating prokaryotic as well as eukaryotic DNA witnessed a remarkable development during the later part of the 20th century. These techniques involved breakage of a DNA molecule at two desired places to isolate a specific DNA segment and its insertion in another DNA molecule at a desired position. The product thus obtained is called recombinant DNA and the technique often called genetic engineering.

Using this technique we can isolate and clone single copy of a gene or a DNA segment into an indefinite number of copies, all identical. This became possible because vectors like plasmids and phages reproduce in a host (e.g. E. coli) in their usual manner even after insertion of foreign DNA, so that the inserted DNA will also replicate faithfully with the parent DNA.

This technique is called gene cloning and the vectors used for this purpose are called cloning vectors. With this technique, using a variety of cloning vectors, genes can be isolated, cloned and characterized, so that the technique has led to significant progress in all areas of molecular biology.

A variety of vectors have also been developed which not only allow multiplication, but may also be manipulated in such a way that the inserted gene may express in the host. Due to the importance of a variety of these cloning and expression vectors in genetic engineering experiments, they are discussed in some detail in this chapter.

.The techniques used for inserting foreign DNA in these vectors and the development of chimeric DNA molecules (for developing molecular probes, gene libraries, etc.)

	Home
BACs and PACs as Vectors for Cloning large DNA Segments (100-300 kb) Bacterial Artificial Chromosomes (BACs) P1-derived Artificial Chromosomes (PACs) Plant and Animal viruses as Vectors Plant Viruses (Cauliflower Mosaic virus or CaMV and Geminiviruses) Animal Viruses Transposons as Vectors Transposons of Higher Plants (Ac/Dc or Mul of Maize) Transposons of drosophila (P elements) YAC and MAC Vectors for Cloning very large DNA Segments (1000 kb) Yeast Artificial Chromosomes (YACs) Mammalian Artificial chromosomes (MACs) Expression Vectors Promoters Nopaline Synthase (nos) Promoter from T-DNA Dual Promoter of Mannopine Synthase (mas) genes 1 and 2 35S Promoter of CaMV Polyhedrin Promoter from Baculovirus Expression Cassettes Baculovirus and Expression Vector System for Insect Cells Virus Expression Vectors for Mammalian Cells Binary and Shuttle Vectors