Agrobacterium Plasmid Vectors, Potential Vector, Foreign DNA, Modified Hybrid, Agrobacterium Infection, Agrobacterium Infects

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Cloning and Expression Vectors Cloning and Expression Vectors Introduction Cloning Vectors for Recombinant DNA Plasmids as Vectors Three Phases of the Development of Plasmid Cloning Vectors Alpha-Complementation for Screening Using "blue-white"Clonies Nonsense Suppressors to Complement Termination Codons in Vectors E.Coli Plasmid Vectors pBR322 and pBr327 Vectors pUC Vectors pSP64-pSP65 pGEM-3, pGEM-4, pGEM-3Z, pGEM-4Z ΠAN13 from ΠAN7 Yeast Plasmid Vectors Retriever Vectors Agrobacterium Plasmid Vectors Lambda Phage (λ)Vectors Lambda Phage (Lysis and Lysogeny) Genetic Markers for the Selection and Screening of λ Vectors Development of Lambda (λ)Phage Vectors Insertion λ Vectors Replacement λ Vectors M13 Phage as a Vector for DNA Sequencing M13-vectors-of-mp-Series Hosts for M13 Vectors Cosmids as Vectors Phagemids as Vectors Phagemids pUC118 and pUC119 Phagemid pBluesript IIKS

Home >> Biotechnology and Genomics >> Cloning and Expression Vectors >> Agrobacterium Plasmid Vectors

Agrobacterium plasmid vectors:

Ti and Ri plasmid vectors for higher plants. Among higher plants, Ti plasmid of Agrobacterium tumifaciens or Ri plasmid of A. rhizogenes is the best known vector. T-DNA from Ti or Ri plasmid of Agrobacterium, is considered to be a very potential vector for cloning experiments with higher plants.

This will involve the following steps: (i) foreign DNA has to be first cloned into T-DNA of Ti or Ri plasmid; (ii) modified; hybrid T -DNA can be transferred to the genome of plant cells by Agrobacterium infection.

Derivation of Recombinant

Derivation of Recombinant Ti plasmid and its use in transformation of higher plants

Derivation of Recombinant Ti plasmid and its use in transformation of higher plants

This system can be widely used, since Agrobacterium infects nearly all dicotyledonous plants. Such cloning in plants has proved to be of immense use to modify agricultural plants to increase their productivity.

Monocotyledonous plants to which our cereal crops belong are, however, difficult materials to be used for improvement through Agrobacterium mediated transformation; success, however, has been achieved in cereals also in recent years.

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