Western Blotting, Detect Proteins, transformed Cells, gene Expresses, Extacted Proteins, Gel Elertrophoresis, Polycrylamide, Nitrocellulose Membrane

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Chimeric DNA Molecular Probes and Gene Libraries Chimeric DNA Molecular Probes and Gene Libraries Introduction Restriction Enzymes for Cloning Technique of Restriction Mapping Restriction Clevage and Gel Electrophoresis Construction of a Restriction Map Use of Partial Digests end Labelling and Hybridization in Restriction Mapping Construction of Chimeric DNA Cloning of Plasmid Vectors Directional Cloning of DNA Fragments with Heterologus Protruding Termini Adding Poly dA at 3' Ends of DNA Clone Blunt End Ligation by T4 DNA Ligase Cloning in Phage λ and Cosmid Vectors Cloning in phage λ Vectors Cloning in Cosmid Vectors Hosts for Cloning Vectors Bacteria as Hosts Eukaryotes as Hosts Molecular Probes Preparation of Probes Genomic DNA Probes

Home >> Biotechnology and Genomics >> Chimeric DNA Molecular Probes and Gene Libraries >> Western blotting

Your Ad Here	Western Blotting This technique is used to detect proteins of a particular specificity. Particularly when a transferred gene expresses in transformed cells, the translated product in the form of protein can be identified by this technique. The extracted proteins are subjected to polyacrylamide gel electrophoresis (PAGE) and are then transferred on to nitrocellulose, to which they bind.
Nitrocellulose membrane is then used for probing with a specific labelled antibody (antibody will not hybridize with protein, but bind with it). The antibody may be labelled with ¹²⁵1 and the signal is detected again with autoradiography. If radioactive label is not used, bound antibody may be detected by a second antibody tagged with an enzyme.

	Home
c DNA Probes Synthetic Oligonucleotides as Probes RNA Probes or Riboprobes Labelling of Probes Radiolablled Probes Non Radioactive Probes Biotin Labelled Probes Digoxigenin Labelled Probes Alternatives to Biotin and Digoxigenin Labelling Amplification of DNA Probe Signals Techniques Used in Molecular Probing Southern Nothern and Western Blotting Southern Blotting Northern Blotting Western Blotting Dot Blots and Slot Blots Applications of Molecular Probes Construction and Screening of Genomic and cDNA Libraries Genomic Library by Shotgun Experiment cDNA Library from mRNAs Colony or Plaque Hybridization for Screening of Libraries Chromosome Walking and Characterization of Chromosome Segments Chromosome Jumping or Hopping Libraries BAC Libraries and Assembly of BACs into Contigs

Language >> English | Français | Español | Deutsch | Italiano | Portugues | Japanese | Korean | Sim-Chinese