Technique of Restriction Mapping, Nature Mutants, Chromosomes, Genetic Background, Genes Genetic Maps, Non Availability Mutants

	Home
Chimeric DNA Molecular Probes and Gene Libraries Chimeric DNA Molecular Probes and Gene Libraries Introduction Restriction Enzymes for Cloning Technique of Restriction Mapping Restriction Clevage and Gel Electrophoresis Construction of a Restriction Map Use of Partial Digests end Labelling and Hybridization in Restriction Mapping Construction of Chimeric DNA Cloning of Plasmid Vectors Directional Cloning of DNA Fragments with Heterologus Protruding Termini Adding Poly dA at 3' Ends of DNA Clone Blunt End Ligation by T4 DNA Ligase Cloning in Phage λ and Cosmid Vectors Cloning in phage λ Vectors Cloning in Cosmid Vectors Hosts for Cloning Vectors Bacteria as Hosts Eukaryotes as Hosts Molecular Probes Preparation of Probes Genomic DNA Probes

Home >> Biotechnology and Genomics >> Chimeric DNA Molecular Probes and Gene Libraries >> Technique of Restriction Mapping

Your Ad Here	Technique of Restriction Mapping Genetic maps are prepared using recombination frequencies. However, the recombination frequencies, which are the function of distances between genes, are not completely independent of (i) the nature' of mutants used, (ii) the position of these mutants on chromosomes, (Hi) the genetic background, (iv) the environmental conditions, and (v) a variety of other factors. Consequently, the distances between genes on a genetic map may not correspond to the distances between them on the DNA molecule of which they are a part at the molecular level.
Gaps may also be present on a genetic map due to non-availability of mutants in that region.At the molecular level the fine structure of a gene can be studied through determination of nucleotide sequence of the concerned DNA segment. Sometimes this is done through isolation of a DNA segment corresponding to a gene followed by sequencing of the DNA segment, which needs time and energy. Instead we can prepare a map of the DNA by its cleavage at specific sites with the help of restriction endonucleases, which recognize very short specific DNA sequences and cleave the DNA at these specific sites. These sites of cleavage can be identified and mapped to give rise to a restriction map.
A restriction map thus consists of a linear sequence of sites, each' tor a specific enzyme and the distances between them are measured in terms of number of base pairs of DNA. This technique can be used both in prokaryotes and eukaryotes, although all regions of chromosomes can not be easily mapped in eukaryotes.	Your Ad Here

	Home
c DNA Probes Synthetic Oligonucleotides as Probes RNA Probes or Riboprobes Labelling of Probes Radiolablled Probes Non Radioactive Probes Biotin Labelled Probes Digoxigenin Labelled Probes Alternatives to Biotin and Digoxigenin Labelling Amplification of DNA Probe Signals Techniques Used in Molecular Probing Southern Nothern and Western Blotting Southern Blotting Northern Blotting Western Blotting Dot Blots and Slot Blots Applications of Molecular Probes Construction and Screening of Genomic and cDNA Libraries Genomic Library by Shotgun Experiment cDNA Library from mRNAs Colony or Plaque Hybridization for Screening of Libraries Chromosome Walking and Characterization of Chromosome Segments Chromosome Jumping or Hopping Libraries BAC Libraries and Assembly of BACs into Contigs