Southern, Northern and Western Blotting, RNA Protein Fragments, Gel Electrophoresis, Molecular Probe, Hybridization, Hybridize

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Chimeric DNA Molecular Probes and Gene Libraries Chimeric DNA Molecular Probes and Gene Libraries Introduction Restriction Enzymes for Cloning Technique of Restriction Mapping Restriction Clevage and Gel Electrophoresis Construction of a Restriction Map Use of Partial Digests end Labelling and Hybridization in Restriction Mapping Construction of Chimeric DNA Cloning of Plasmid Vectors Directional Cloning of DNA Fragments with Heterologus Protruding Termini Adding Poly dA at 3' Ends of DNA Clone Blunt End Ligation by T4 DNA Ligase Cloning in Phage λ and Cosmid Vectors Cloning in phage λ Vectors Cloning in Cosmid Vectors Hosts for Cloning Vectors Bacteria as Hosts Eukaryotes as Hosts Molecular Probes Preparation of Probes Genomic DNA Probes

Home >> Biotechnology and Genomics >> Chimeric DNA Molecular Probes and Gene Libraries >> Southern - Northern and Western Blotting

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Southern, Northern and Western blotting

A mixture of DNA, RNA or protein fragments can be separated by gel electrophoresis and the separated bands can be stained and visualized directly in the gel. However, to confirm the identity of these bands or to find similarity of one or more of these bands with a known and available molecular probe, it is possible to hybridize these bands with a labelled probe.

To facilitate this hybridization, the bands are often transferred to a nitrocellulose membrane through a technique described as blotting. When DNA bands are thus blotted, this is called Southern blotting (after the name of' E.M. Southern); when RNA bands are thus transferred it is described by the jargon term Northern blotting and similarly when protein bands are transferred, the technique is describe as Western blotting.

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c DNA Probes Synthetic Oligonucleotides as Probes RNA Probes or Riboprobes Labelling of Probes Radiolablled Probes Non Radioactive Probes Biotin Labelled Probes Digoxigenin Labelled Probes Alternatives to Biotin and Digoxigenin Labelling Amplification of DNA Probe Signals Techniques Used in Molecular Probing Southern Nothern and Western Blotting Southern Blotting Northern Blotting Western Blotting Dot Blots and Slot Blots Applications of Molecular Probes Construction and Screening of Genomic and cDNA Libraries Genomic Library by Shotgun Experiment cDNA Library from mRNAs Colony or Plaque Hybridization for Screening of Libraries Chromosome Walking and Characterization of Chromosome Segments Chromosome Jumping or Hopping Libraries BAC Libraries and Assembly of BACs into Contigs