Probes or Riboprobes, DNA Templates, RNA Synthesized, Specific RNA Polymerase, Hybridization Blots, Ribonucleases, Bare Hands

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Chimeric DNA Molecular Probes and Gene Libraries Chimeric DNA Molecular Probes and Gene Libraries Introduction Restriction Enzymes for Cloning Technique of Restriction Mapping Restriction Clevage and Gel Electrophoresis Construction of a Restriction Map Use of Partial Digests end Labelling and Hybridization in Restriction Mapping Construction of Chimeric DNA Cloning of Plasmid Vectors Directional Cloning of DNA Fragments with Heterologus Protruding Termini Adding Poly dA at 3' Ends of DNA Clone Blunt End Ligation by T4 DNA Ligase Cloning in Phage λ and Cosmid Vectors Cloning in phage λ Vectors Cloning in Cosmid Vectors Hosts for Cloning Vectors Bacteria as Hosts Eukaryotes as Hosts Molecular Probes Preparation of Probes Genomic DNA Probes

Home >> Biotechnology and Genomics >> Chimeric DNA Molecular Probes and Gene Libraries >> RNA Probes or Riboprobes

RNA probes or riboprobes

High specific activity RNA probes or riboprobes may also be synthesized from DNA templates cloned in expression vectors. This is achieved through RNA synthesized in vitro (on DNA inserted in vector) and labelled simultaneously employing specific RNA polymerase and rNTPs, which are supplied by commercial firms in a kit for riboprobe preparation.

RNA probes offer several advantages over DNA probes, since these are single stranded, and provide improved signal or hybridization blots. However, widespread presence of ribonucleases creates some problems in their preparation and use, because these ribonucleases will cause degradation of RNA which is synthesized.

Therefore, extreme care is needed in the preparation of riboprobe, keeping all glassware ribonuclease free (no glassware should be touched with bare hands, because ribonucleases may be available on our skin).

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c DNA Probes Synthetic Oligonucleotides as Probes RNA Probes or Riboprobes Labelling of Probes Radiolablled Probes Non Radioactive Probes Biotin Labelled Probes Digoxigenin Labelled Probes Alternatives to Biotin and Digoxigenin Labelling Amplification of DNA Probe Signals Techniques Used in Molecular Probing Southern Nothern and Western Blotting Southern Blotting Northern Blotting Western Blotting Dot Blots and Slot Blots Applications of Molecular Probes Construction and Screening of Genomic and cDNA Libraries Genomic Library by Shotgun Experiment cDNA Library from mRNAs Colony or Plaque Hybridization for Screening of Libraries Chromosome Walking and Characterization of Chromosome Segments Chromosome Jumping or Hopping Libraries BAC Libraries and Assembly of BACs into Contigs

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