Northern Blotting, Technique Southern Blotting, Blot Transfer RNA, Chemically Reactive Paper, Diazotization, Aminobenzyloxymethyl Paper

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Chimeric DNA Molecular Probes and Gene Libraries Chimeric DNA Molecular Probes and Gene Libraries Introduction Restriction Enzymes for Cloning Technique of Restriction Mapping Restriction Clevage and Gel Electrophoresis Construction of a Restriction Map Use of Partial Digests end Labelling and Hybridization in Restriction Mapping Construction of Chimeric DNA Cloning of Plasmid Vectors Directional Cloning of DNA Fragments with Heterologus Protruding Termini Adding Poly dA at 3' Ends of DNA Clone Blunt End Ligation by T4 DNA Ligase Cloning in Phage λ and Cosmid Vectors Cloning in phage λ Vectors Cloning in Cosmid Vectors Hosts for Cloning Vectors Bacteria as Hosts Eukaryotes as Hosts Molecular Probes Preparation of Probes Genomic DNA Probes

Home >> Biotechnology and Genomics >> Chimeric DNA Molecular Probes and Gene Libraries >> Northern blotting

Your Ad Here

Northern Blotting

Initially the technique of Southern blotting used for DNA transfer from gel to the membrane could not be used for blot-transfer of RNA. Instead mRNA bands from the gel were blot-transferred onto a chemically reactive paper, prepared by diazotization of aminobenzyloxymethyl paper.

The technique being related to Southern blotting was called Northern blotting (not after the name of any scientist as in Southern blotting).

Later, it was shown that mRNA bands can be hybridized with a labelled DNA or RNA probe.

The single stranded regions of the hybridized probe are removed by nuclease (mungbean nuclease or S-l nuclease), so that quantitative estimation of hybridized mRNA can also be made.

	Home
c DNA Probes Synthetic Oligonucleotides as Probes RNA Probes or Riboprobes Labelling of Probes Radiolablled Probes Non Radioactive Probes Biotin Labelled Probes Digoxigenin Labelled Probes Alternatives to Biotin and Digoxigenin Labelling Amplification of DNA Probe Signals Techniques Used in Molecular Probing Southern Nothern and Western Blotting Southern Blotting Northern Blotting Western Blotting Dot Blots and Slot Blots Applications of Molecular Probes Construction and Screening of Genomic and cDNA Libraries Genomic Library by Shotgun Experiment cDNA Library from mRNAs Colony or Plaque Hybridization for Screening of Libraries Chromosome Walking and Characterization of Chromosome Segments Chromosome Jumping or Hopping Libraries BAC Libraries and Assembly of BACs into Contigs