Dot Blots and Slot Blots, Southern Northern Blots, Pure Extracted DNAs, Nitrocellulose Membrane, DNA Blots,Immobilized Denatured

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Chimeric DNA Molecular Probes and Gene Libraries Chimeric DNA Molecular Probes and Gene Libraries Introduction Restriction Enzymes for Cloning Technique of Restriction Mapping Restriction Clevage and Gel Electrophoresis Construction of a Restriction Map Use of Partial Digests end Labelling and Hybridization in Restriction Mapping Construction of Chimeric DNA Cloning of Plasmid Vectors Directional Cloning of DNA Fragments with Heterologus Protruding Termini Adding Poly dA at 3' Ends of DNA Clone Blunt End Ligation by T4 DNA Ligase Cloning in Phage λ and Cosmid Vectors Cloning in phage λ Vectors Cloning in Cosmid Vectors Hosts for Cloning Vectors Bacteria as Hosts Eukaryotes as Hosts Molecular Probes Preparation of Probes Genomic DNA Probes

Home >> Biotechnology and Genomics >> Chimeric DNA Molecular Probes and Gene Libraries >> Dot blots and slot blots

Your Ad Here	Dot blots and slot blots Another variety of Southern or Northern blots is dot blots or slot blots. In dot blots, cloned or pure extracted DNAs to be tested are spotted adjacent to each other on a nitrocellulose membrane. DNA blots thus produced are immobilized and denatured so that they are bound on the membrane as single stranded DNA blots. The membrane is then hybridized with readioactively labelled probe (DNA or RNA).
The dot representing sequence related to probe will light up by autoradiography The intensity of the dot will indicate the relative concentration of sequence related to the probe in the DNA sample used for a particular dot. No steps involving digestion with enzyme, gel electrophoresis or transfer from gel to membrane are needed in this technique. Therefore, it is considered much more convenient, when no restriction sites are needed to be studied.
Sometimes, an apparatus is used for placing spots on the membranes, through slots made in this equipment. The spots made thus are in the form of oblong slots rather than round dots. These slots are used just like dot blots and are described as slot blots	Your Ad Here

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c DNA Probes Synthetic Oligonucleotides as Probes RNA Probes or Riboprobes Labelling of Probes Radiolablled Probes Non Radioactive Probes Biotin Labelled Probes Digoxigenin Labelled Probes Alternatives to Biotin and Digoxigenin Labelling Amplification of DNA Probe Signals Techniques Used in Molecular Probing Southern Nothern and Western Blotting Southern Blotting Northern Blotting Western Blotting Dot Blots and Slot Blots Applications of Molecular Probes Construction and Screening of Genomic and cDNA Libraries Genomic Library by Shotgun Experiment cDNA Library from mRNAs Colony or Plaque Hybridization for Screening of Libraries Chromosome Walking and Characterization of Chromosome Segments Chromosome Jumping or Hopping Libraries BAC Libraries and Assembly of BACs into Contigs

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