Biotechnology and Metabolic Engineering, Production of Metabolites, Optimized for Industrial, Regulatory Elements, Genetic Manipulations

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Biotechnology and metabolic engineering

One of the major objectives of biotechnology research is the use of living. systems for the production of metabolites at the industrial scale. However, cell's metabolic networks that evolved in nature are not optimized for industrial production of these metabolites. In such cases performance of metabolic pathways are being manipulated, so that the metabolites are overproduced. The opportunity to introduce heterologous genes and regulatory elements made 'metabolic engineering', a very fascinating area of research.

However, there is a variety of limitations in metabolic engineering that need to be overcome. For instance, when alterations are made (by genetic manipulations) for flux distributions at key branch points (called nodes) of a metabolic pathway, this may be opposed by mechanisms evolved in the cell for its optimal growth. This is described as 'network rigidity' and efforts are being made to overcome problems like this.

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